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从野鸡上呼吸道中分离和鉴定产蛋白酶细菌。

Isolation and characterization of protease producing bacteria from upper respiratory tract of wild chicken.

作者信息

Raut Sangeeta, Sen Sudip Kumar, Kabir Naveed Alam, Satpathy Soumya, Raut Smita

出版信息

Bioinformation. 2012;8(7):326-30. doi: 10.6026/97320630008326. Epub 2012 Apr 13.

DOI:10.6026/97320630008326
PMID:22553390
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3338977/
Abstract

Bacterial samples isolated from the upper respiratory tract of a healthy broiler chicken and a wild chicken suffering from influenza which were collected locally revealed proteolytic activity as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and zymogram analysis. Among five protease producing strains screened, one was selected as promising protease producer. The activity of the protease produced by this organism is stable up to 620C. Optimum yield was achieved after 19 hours of culture, at pH 9.0 and 450C. The desired protein was precipitated from the crude extract by using ammonium sulfate (60%) followed by dialysis and purified by Ion-exchange chromatography. Further investigations are needed to know about the structure elucidation of the purified protein for industrial exploitation.

摘要

从当地收集的一只健康肉鸡和一只患流感的野鸡的上呼吸道中分离出的细菌样本显示，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和酶谱分析检测到其具有蛋白水解活性。在筛选出的五株产蛋白酶菌株中，选择了一株作为有前景的蛋白酶产生菌。该菌株产生的蛋白酶活性在62℃时仍保持稳定。在pH 9.0和45℃下培养19小时后可获得最佳产量。通过使用硫酸铵（60%）从粗提物中沉淀出所需蛋白质，然后进行透析，并通过离子交换色谱法进行纯化。为了进行工业开发，还需要进一步研究以了解纯化蛋白质的结构解析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c62/3338977/5e6fce37a3f4/97320630008326F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c62/3338977/b02f17875834/97320630008326F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c62/3338977/a85223a1733c/97320630008326F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c62/3338977/757557cdf34d/97320630008326F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c62/3338977/5e6fce37a3f4/97320630008326F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c62/3338977/b02f17875834/97320630008326F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c62/3338977/a85223a1733c/97320630008326F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c62/3338977/757557cdf34d/97320630008326F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c62/3338977/5e6fce37a3f4/97320630008326F4.jpg

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