Marsh A, Evans M B, Strang J
Pathology Department, Bethlem Royal Hospital, Beckenham, Kent, UK.
J Pharm Biomed Anal. 1995 Jun;13(7):829-39. doi: 10.1016/0731-7085(95)01517-o.
This communication addresses the analytical problems associated with the analysis of hair specimens from known users and misusers of the synthetic opioid methadone. An adapted radioimmunoassay and a previously developed preanalytical decontamination procedure have been applied to samples from known drug users. The removal of drugs from the hair surface by washing and the effect of proprietary hair treatments on methadone entrapped in the hair have also been investigated. Pre-analytical washing reduced methadone levels by up to 29%, whilst hair colouration and peroxide bleaching were found to reduce levels by up to 21% and 50% respectively. Methadone assay of extracts from dated hair segments were shown to provide long-term histories of methadone intake, under controlled and non-controlled conditions. Evidence that a dose relationship between hair drug levels and intake may exist is presented. Results of hair analysis, expressed as ng methadone/mg hair, from drug users (range 0.20-10.63) are compared to a pre-determined cut-off of 0.1 ng methadone/mg hair, obtained from the analysis of a known drug free population (n = 23).
本通讯探讨了与分析合成阿片类药物美沙酮已知使用者和滥用者毛发样本相关的分析问题。一种改良的放射免疫分析法和先前开发的分析前净化程序已应用于已知吸毒者的样本。还研究了通过洗涤从毛发表面去除药物以及专用毛发处理对毛发中截留的美沙酮的影响。分析前洗涤可使美沙酮水平降低多达29%，而染发和用过氧化氢漂白分别可使美沙酮水平降低多达21%和50%。结果表明，对不同时期毛发段提取物进行美沙酮检测，可在受控和非受控条件下提供美沙酮摄入的长期记录。本文提供了毛发药物水平与摄入量之间可能存在剂量关系的证据。将吸毒者毛发分析结果（以纳克美沙酮/毫克毛发表示，范围为0.20 - 10.63）与从已知无毒品人群（n = 23）分析中获得的预先确定的0.1纳克美沙酮/毫克毛发的临界值进行了比较。
