Cañete-Soler R, Gui Y H, Linask K K, Muschel R J
Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Dev Dyn. 1995 Sep;204(1):30-40. doi: 10.1002/aja.1002040105.
Considerable remodeling of the extracellular matrix as well as cellular migration takes place during embryogenesis. Since the metalloproteinase MMP-9 is implicated in these functions in cancer cells, we studied the patterns of expression of MMP-9 mRNA during the development of post-implantation mouse embryos. MMP-9 mRNA was detected using the ribonuclease protection assay in poly A+RNA from 13 to 17 day embryos, but not at 11 days. In order to localize these transcripts, in situ hybridization was performed on sections of murine embryos from 7.5 to 15 days of gestation. At the time of implantation, MMP-9 mRNA was localized to the invading trophoblast cells. Strong signals were also seen in the yolk sac. No signal for MMP-9 mRNA was seen by in situ hybridization in the embryo until day 11 when detectable reaction was seen in the central nervous system. By day 15 strong signals were seen in the liver, in the developing bronchial epithelium of the lungs and in the primordial alveoli, in the epithelium of the thyroid gland, in the thymus, in the endochondrial plates of the bone, and in neural cells. The liver from day 15 embryos contained gelatinase activity at 105 kDa consistent with MMP-9. Thus, MMP-9 expression appears to be expressed in specific organs in a precise temporal sequence during development.
在胚胎发育过程中，细胞外基质会发生显著重塑以及细胞迁移。由于金属蛋白酶MMP - 9在癌细胞的这些功能中发挥作用，我们研究了植入后小鼠胚胎发育过程中MMP - 9 mRNA的表达模式。使用核糖核酸酶保护分析法在13至17天胚胎的多聚A + RNA中检测到MMP - 9 mRNA，但在11天时未检测到。为了定位这些转录本，对妊娠7.5至15天的小鼠胚胎切片进行原位杂交。在植入时，MMP - 9 mRNA定位于侵入的滋养层细胞。在卵黄囊中也观察到强信号。直到第11天，在胚胎中通过原位杂交未观察到MMP - 9 mRNA的信号，此时在中枢神经系统中观察到可检测的反应。到第15天，在肝脏、肺发育中的支气管上皮、原始肺泡、甲状腺上皮、胸腺、骨的软骨内板和神经细胞中观察到强信号。来自15天胚胎的肝脏含有与MMP - 9一致的105 kDa明胶酶活性。因此，在发育过程中，MMP - 9的表达似乎在特定器官中按精确的时间顺序表达。
