Coss R A, Alden M E, Wachsberger P R, Smith N N
Department of Radiation Oncology, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Int J Radiat Oncol Biol Phys. 1996 Jan 15;34(2):403-10. doi: 10.1016/0360-3016(95)02039-x.
The response of the microtubular (MT) cytoskeleton to hyperthermia was assessed as a prognostic indicator of cytotoxicity.
Heat-induced collapse and subsequent recovery of the MT system were compared with survival for both nonthermotolerant (NT) and thermotolerant (TT) G1 populations of Chinese hamster ovary (CHO) cells. The response of the MT system was monitored using immunofluorescence staining. The G1 populations of NT and TT cells were heated by submersion in 45.0 and 43.0 degrees C waterbaths.
Heat-induced perinuclear collapse of the MT system did not correlate with survival for the NT and TT populations. However, recovery of the organization of the MT cytoskeleton was correlatable with survival. The regression line of survival plotted as a function of MT recovery is fit by: y = -0.43 + 1.03x, r2 = 0.95 (p < 0.0005).
Restoration of the organization of the MT cytoskeleton following hyperthermia may be used as a prognostic indicator of survival of CHO cells heated in G1.
评估微管（MT）细胞骨架对热疗的反应，作为细胞毒性的预后指标。
将热诱导的MT系统的崩溃及随后的恢复情况与中国仓鼠卵巢（CHO）细胞的非热耐受（NT）和热耐受（TT）G1群体的存活率进行比较。使用免疫荧光染色监测MT系统的反应。通过将NT和TT细胞的G1群体浸入45.0和43.0摄氏度的水浴中来加热。
热诱导的MT系统的核周崩溃与NT和TT群体的存活率无关。然而，MT细胞骨架组织的恢复与存活率相关。以MT恢复为函数绘制的存活回归线拟合为：y = -0.43 + 1.03x，r2 = 0.95（p < 0.0005）。
热疗后MT细胞骨架组织的恢复可作为G1期加热的CHO细胞存活的预后指标。
