Liu J, Nagy Z, Joris H, Tournaye H, Smitz J, Camus M, Devroey P, Van Steirteghem A
Centre for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University, Belgium.
Hum Reprod. 1995 Oct;10(10):2630-6.
From October 1992 to December 1994, 2732 cycles of treatment by intracytoplasmic sperm injection (ICSI) were carried out in couples mainly with severe male-factor infertility. The overall fertilization rate in these 2732 cycles was 71% of intact oocytes. However, in 76 (72 couples) of these cycles, none of the injected oocytes became fertilized, so the total fertilization failure rate was 3% (76/2732 cycles). Details of these 76 cycles were analysed. The results show that total fertilization failure after ICSI may be explained by different factors related to (i) semen characteristics (only immotile or round-headed spermatozoa for ICSI) or (ii) the oocytes (number, abnormal morphology, damage after ICSI). Of 26 couples, 22 achieved fertilization in their subsequent ICSI cycles. In conclusion, total fertilization failure after ICSI for the treatment of severe male-factor infertility was mainly caused by the poor viability of the spermatozoa used for injection; it was also associated with a low number and poor quality of oocytes. Repeated ICSI treatment may be useful or necessary in couples with total fertilization failure.
1992年10月至1994年12月，主要针对严重男性因素不育的夫妇进行了2732个周期的卵胞浆内单精子注射（ICSI）治疗。在这2732个周期中，完整卵母细胞的总体受精率为71%。然而，在其中76个（72对夫妇）周期中，注射的卵母细胞均未受精，因此总受精失败率为3%（76/2732个周期）。对这76个周期的详细情况进行了分析。结果表明，ICSI后总受精失败可能由与以下方面相关的不同因素解释：（i）精液特征（仅用于ICSI的不动或圆头精子）或（ii）卵母细胞（数量、形态异常、ICSI后的损伤）。在26对夫妇中，22对在随后的ICSI周期中实现了受精。总之，ICSI治疗严重男性因素不育后总受精失败主要是由于用于注射的精子活力差所致；也与卵母细胞数量少和质量差有关。对于总受精失败的夫妇，重复ICSI治疗可能是有用的或必要的。
