三种浓缩技术对从牛粪中回收的微小隐孢子虫卵囊活力的影响。
Effect of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces.
作者信息
Bukhari Z, Smith H V
机构信息
Scottish Parasite Diagnostic Laboratory, Stobhill NHS Trust, Springburn, Glasgow, Scotland.
出版信息
J Clin Microbiol. 1995 Oct;33(10):2592-5. doi: 10.1128/jcm.33.10.2592-2595.1995.
Abstract
Bovine fecal samples (1 g) negative for Cryptosporidium sp. oocysts were seeded with 7 x 10(4) Cryptosporidium parvum oocysts and purified by either water-ether concentration, sucrose density flotation, or zinc sulfate flotation to evaluate oocyst recovery. The effect of these purification techniques on the viability of recovered oocysts was also evaluated. Significantly higher numbers of seeded oocysts were recovered by water-ether concentration (recovery rate, 46 to 75%) than by sucrose density (24 to 65%) or zinc sulfate (22 to 41%) flotation methods. In addition, water-ether concentration did not exert a significant effect on the viability of the population of oocysts recovered, whereas sucrose density flotation and zinc sulfate flotation selectively concentrated viable oocysts. The water-ether concentration procedure is recommended for use in epidemiological studies in which both oocyst enumeration and viability assessment are required.
摘要
将不含隐孢子虫属卵囊的牛粪便样本（1克）接种7×10⁴个微小隐孢子虫卵囊，然后通过水 - 乙醚浓缩法、蔗糖密度浮选法或硫酸锌浮选法进行纯化，以评估卵囊回收率。还评估了这些纯化技术对回收卵囊活力的影响。通过水 - 乙醚浓缩法回收的接种卵囊数量（回收率为46%至75%）显著高于蔗糖密度浮选法（24%至65%）或硫酸锌浮选法（22%至41%）。此外，水 - 乙醚浓缩法对回收的卵囊群体活力没有显著影响，而蔗糖密度浮选法和硫酸锌浮选法选择性地富集了有活力的卵囊。对于需要同时进行卵囊计数和活力评估的流行病学研究，建议使用水 - 乙醚浓缩法。
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