Mtambo M M, Nash A S, Blewett D A, Wright S
Department of Veterinary Medicine, Glasgow University Veterinary School, Bearsden, UK.
Vet Parasitol. 1992 Dec;45(1-2):49-57. doi: 10.1016/0304-4017(92)90026-6.
Modified Ziehl-Neelsen (MZN), auramine-phenol (A-P) and fluorescein isothiocyanate-labelled (FITC-labelled) monoclonal antibody (MAb) techniques were compared for detection of Cryptosporidium parvum oocysts in cat faecal specimens inoculated with known numbers of C. parvum oocysts. Of the three techniques, the FITC-labelled MAb technique detected more oocysts than the MZN and A-P techniques (P < 0.05), but A-P was more efficient than MZN (P < 0.05). Comparison of sucrose flotation, zinc sulphate (ZnSO4) flotation and formol-ether (F-E) sedimentation techniques revealed that F-E was the most efficient of the three (P < 0.05) for concentration of C. parvum oocysts from cat faecal specimens. On average, the F-E technique recovered 37% of oocysts from the original sample, whereas the sucrose and ZnSO4 flotation techniques recovered 33% and 11%, respectively. The findings of this study suggest that MZN and A-P staining are both useful for screening C. parvum oocysts in cat faecal materials containing 10(6) oocysts or more, but FITC-labelled MAb should be used when the number of oocysts is low. Also, the F-E sedimentation technique is recommended for concentrating oocysts in cat faecal specimens.
对改良齐-尼氏染色法（MZN）、金胺-酚染色法（A-P）和异硫氰酸荧光素标记（FITC标记）单克隆抗体（MAb）技术进行了比较，以检测接种已知数量微小隐孢子虫卵囊的猫粪便标本中的微小隐孢子虫卵囊。在这三种技术中，FITC标记的MAb技术检测到的卵囊比MZN和A-P技术更多（P<0.05），但A-P比MZN更有效（P<0.05）。对蔗糖漂浮法、硫酸锌（ZnSO4）漂浮法和甲醛-乙醚（F-E）沉淀法的比较显示，F-E是从猫粪便标本中浓缩微小隐孢子虫卵囊的三种方法中最有效的（P<0.05）。平均而言，F-E技术从原始样本中回收了37%的卵囊，而蔗糖和ZnSO4漂浮法分别回收了33%和11%。本研究结果表明，MZN和A-P染色法对于筛查含有10^6个或更多卵囊的猫粪便材料中的微小隐孢子虫卵囊均有用，但当卵囊数量较少时应使用FITC标记的MAb。此外，推荐使用F-E沉淀法浓缩猫粪便标本中的卵囊。
