用于测定1型人类免疫缺陷病毒齐多夫定耐药性基因型相对含量的非同位素杂交检测法
Nonisotopic hybridization assay for determination of relative amounts of genotypic human immunodeficiency virus type 1 zidovudine resistance.
作者信息
Eastman P S, Boyer E, Mole L, Kolberg J, Urdea M, Holodniy M
机构信息
Chiron Corporation, Emeryville, California 94608, USA.
出版信息
J Clin Microbiol. 1995 Oct;33(10):2777-80. doi: 10.1128/jcm.33.10.2777-2780.1995.
Abstract
A nonisotopic hybridization assay for human immunodeficiency virus genotypic zidovudine resistance determination is described. Biotinylated PCR product was hybridized with enzyme-labeled probes for wild-type or resistant mutant sequences and detected colorimetrically or chemiluminescently in a microplate format. Changes in mutant-to-wild-type ratios allow for the monitoring of longitudinal patient samples.
摘要
描述了一种用于确定人类免疫缺陷病毒基因型齐多夫定耐药性的非同位素杂交检测方法。生物素化的聚合酶链反应（PCR）产物与针对野生型或耐药突变序列的酶标记探针杂交，并以微孔板形式进行比色或化学发光检测。突变型与野生型比例的变化有助于监测患者的纵向样本。
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