Clavel F, Hoggan M D, Willey R L, Strebel K, Martin M A, Repaske R
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
J Virol. 1989 Mar;63(3):1455-9. doi: 10.1128/JVI.63.3.1455-1459.1989.
We investigated genetic recombination of the human immunodeficiency virus (HIV) in a tissue culture system. A clonal cell line expressing a single integrated HIV provirus with a termination codon affecting pol gene expression was transfected with different defective mutants derived from an infectious molecular clone of HIV. Replication-competent viral particles were recovered, passaged, and plaque purified. Restriction analyses of the proviral DNA corresponding to several of these viruses indicated that their emergence was the result of genetic recombination.
我们在组织培养系统中研究了人类免疫缺陷病毒（HIV）的基因重组。用源自HIV感染性分子克隆的不同缺陷型突变体转染一个表达单一整合HIV前病毒且带有影响pol基因表达的终止密码子的克隆细胞系。回收具有复制能力的病毒颗粒，传代并进行空斑纯化。对与其中几种病毒对应的前病毒DNA进行的限制性分析表明，它们的出现是基因重组的结果。
