在意大利检测到的6株2c型丙型肝炎病毒的部分核苷酸测序
Partial nucleotide sequencing of six subtype 2c hepatitis C viruses detected in Italy.
作者信息
Cammarota G, Maggi F, Vatteroni M L, Da Prato L, Barsanti L, Bendinelli M, Pistello M
机构信息
Department of Biomedicine, University of Pisa, Italy.
出版信息
J Clin Microbiol. 1995 Oct;33(10):2781-4. doi: 10.1128/jcm.33.10.2781-2784.1995.
Abstract
The great majority of 121 hepatitis C virus (HCV) isolates obtained from 117 Italian patients with community-acquired infection could readily be typed by genotype-specific PCR. Subtype 1b was dominant (74 isolates); subtypes 2b, 2a, and 1a followed, with 19, 14, and 8 isolates, respectively. The six isolates that remained untyped by this method were classified as subtype 2c on the basis of sequence analysis of PCR amplicors obtained from the core and NS5 genes. These findings indicate that HCV subtype 2c has a relatively high prevalence in Italy. Sequencing the core region from positions 160 to 259 is sufficient to distinguish subtype 2c from other known HCV genotypes.
摘要
从117名意大利社区获得性感染患者中分离出的121株丙型肝炎病毒（HCV），绝大多数都可以通过基因型特异性聚合酶链反应（PCR）轻松分型。1b亚型占主导地位（74株）；其次是2b、2a和1a亚型，分别有19株、14株和8株。通过这种方法仍未分型的6株病毒，根据从核心基因和NS5基因获得的PCR扩增子的序列分析，被归类为2c亚型。这些发现表明，HCV 2c亚型在意大利的患病率相对较高。对160至259位的核心区域进行测序足以将2c亚型与其他已知的HCV基因型区分开来。
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