Cerino A, Cividini A, Asti M, Lanza A, Silini E, Mondelli M U
Istituto di Clinica delle Malattie Infettive, University of Pavia Medical School, Italy.
J Clin Microbiol. 1996 Mar;34(3):714-6. doi: 10.1128/jcm.34.3.714-716.1996.
Serologic methods of typing for hepatitis C virus offer advantages over PCR-based typing methods in terms of speed and simplicity of sample preparation and in the use of standard laboratory equipment. We examined the sensitivities and specificities of two hepatitis C virus serotyping assays which use sets of type-specific antigenic peptides derived from the core or the nonstructural 4 (NS4) regions and compared the results with those of molecular typing with type-specific primers from the core region. Although there was a good concordance between serotyping by either assay and genotyping, we found that the sensitivities of both serologic assays were less than optimal compared with that of molecular typing, with only about 50% of samples being unequivocally typed. Moreover, amino acid sequence similarities within the regions of the genome used for serotyping preclude differentiation into subtypes, which may have important clinical and therapeutic implications.
丙型肝炎病毒血清学分型方法在样本制备的速度和简便性以及使用标准实验室设备方面比基于聚合酶链反应（PCR）的分型方法具有优势。我们检测了两种丙型肝炎病毒血清学分型检测方法的敏感性和特异性，这两种方法使用源自核心区或非结构4（NS4）区的型特异性抗原肽组，并将结果与使用核心区型特异性引物进行分子分型的结果进行比较。尽管两种检测方法的血清学分型与基因分型之间有良好的一致性，但我们发现，与分子分型相比，两种血清学检测方法的敏感性均未达到最佳，只有约50%的样本能够明确分型。此外，用于血清分型的基因组区域内的氨基酸序列相似性使得无法区分亚型，这可能具有重要的临床和治疗意义。
