Harvey B S, Koeuth T, Versalovic J, Woods C R, Lupski J R
Department of Pediatrics, University of Texas Health Sciences Center, Houston, USA.
Infect Control Hosp Epidemiol. 1995 Oct;16(10):564-9. doi: 10.1086/647009.
To confirm the vertical transmission of Citrobacter diversus from a mother to her infant and to evaluate the epidemiologic usefulness of a new automated procedure for analysis of polymerase chain reaction (PCR)-generated DNA fingerprints.
Repetitive element-based PCR (rep-PCR) analysis of C diversus isolates from the blood and amniotic fluid of a mother and the blood of her infant was performed. Unrelated C diversus isolates also were characterized and compared with the isolates from mother and infant. DNA fingerprints were generated by gel electrophoresis of PCR products derived from either unlabeled standard repetitive sequence-based oligonucleotide primers or fluorescent primers. The standard rep-PCR fingerprints were analyzed by visual inspection. The fluorescent primers were used in fluorophore-enhanced rep-PCR (FERP), and the FERP DNA fingerprints were analyzed by an Applied BioSystems (ABI) Model 373A laser scanning unit equipped with Genescan 672 software (Applied Biosystems, Inc, Foster City, CA).
A mother and her newborn infant, both with invasive disease due to C diversus, in an urban tertiary-care hospital.
The DNA fingerprints of the maternal blood, amniotic fluid, and infant blood isolates of C diversus were identical by both visual inspection of ethidium bromide-stained agarose gels and computer-aided analysis of FERP patterns. These strains appeared to differ from all but one control isolate, which had been collected 7 years earlier in the same city in which the infant was born.
Vertical transmission of C diversus from mother to infant can occur in utero. Automated analysis of rep-PCR-generated DNA fingerprints derived using fluorescent primers is an objective means for comparing isolates of C diversus and in all likelihood would be useful for other species of bacteria that possess repetitive elements.
证实不同种柠檬酸杆菌从母亲垂直传播至其婴儿，并评估一种用于分析聚合酶链反应（PCR）产生的DNA指纹的新型自动化程序的流行病学实用性。
对一名母亲血液和羊水以及其婴儿血液中的不同种柠檬酸杆菌分离株进行基于重复元件的PCR（rep-PCR）分析。还对不相关的不同种柠檬酸杆菌分离株进行了特征鉴定，并与母亲和婴儿的分离株进行比较。DNA指纹通过来自未标记的基于标准重复序列的寡核苷酸引物或荧光引物的PCR产物的凝胶电泳产生。标准rep-PCR指纹通过目视检查进行分析。荧光引物用于荧光增强rep-PCR（FERP），FERP DNA指纹通过配备Genescan 672软件的Applied BioSystems（ABI）373A型号激光扫描装置进行分析（Applied Biosystems公司，加利福尼亚州福斯特城）。
在一家城市三级护理医院中，一名母亲及其新生儿均患有由不同种柠檬酸杆菌引起的侵袭性疾病。
通过溴化乙锭染色琼脂糖凝胶的目视检查以及FERP模式的计算机辅助分析，母亲血液、羊水和婴儿血液中不同种柠檬酸杆菌分离株的DNA指纹均相同。这些菌株似乎与除一个对照分离株外的所有菌株不同，该对照分离株是7年前在婴儿出生的同一城市采集的。
不同种柠檬酸杆菌可在子宫内从母亲垂直传播至婴儿。使用荧光引物对rep-PCR产生的DNA指纹进行自动化分析是比较不同种柠檬酸杆菌分离株的一种客观方法，并且很可能对具有重复元件的其他细菌种类也有用。
