通过X射线微量分析测定有丝分裂染色质中元素的浓度。
Concentration of elements in mitotic chromatin as measured by x-ray microanalysis.
作者信息
Cameron I L, Sparks R L, Horn K L, Smith N R
出版信息
J Cell Biol. 1977 Apr;73(1):193-9. doi: 10.1083/jcb.73.1.193.
Abstract
Unfixed frozen-dried and uncoated tissue sections of the mouse duodenum were placed on carbon planchets and analyzed in a scanning electron microscope fitted with energy dispersive X-ray equipment. Computer analysis of the X-ray spectra allowed elemental microanalysis of the nucleus, cytoplasm, and mitotic chromatin regions in the cryptal and villus enterocytes. The peak to continuum ratio of S, Cl, K, and Ca were higher in mitotic chromatin than any of the other sites measured. The redistribution of Ca at mitosis is postulated to help explain both chromosome condensation and assembly of the mitotic spindle apparatus.
摘要
将未固定、冻干且未包被的小鼠十二指肠组织切片置于碳盘上，在配备能量色散X射线设备的扫描电子显微镜下进行分析。对X射线光谱进行计算机分析，可对隐窝和绒毛肠上皮细胞的细胞核、细胞质及有丝分裂染色质区域进行元素微分析。有丝分裂染色质中硫、氯、钾和钙的峰谷比高于所测量的其他任何部位。据推测，有丝分裂时钙的重新分布有助于解释染色体凝聚和有丝分裂纺锤体装置的组装。
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