Hayase Y, Uno F, Nii S
Department of Virology, Jichi Medical School, Tochigi, Japan.
J Electron Microsc (Tokyo). 1995 Oct;44(5):281-8.
Ultrahigh-resolution scanning electron microscopy of MDCK cells infected with influenza viruses was carried out by the uncoated uranyl acetate staining preparation method. Ridge-like protrusions were detected on virus particles and were considered to be an indication of aggregated glycoprotein spikes. Bundles of filamentous virus particles along with bacillary virus particles were encountered on MDCK cells infected with freshly isolated strains of type A virus. Filamentous virus particles that were twisted like ropes were observed on MDCK cells infected with strains of type B virus.
采用未包被醋酸铀染色制片法对感染流感病毒的MDCK细胞进行超高分辨率扫描电子显微镜观察。在病毒颗粒上检测到脊状突起，认为这是糖蛋白刺突聚集的表现。在感染新分离甲型病毒株的MDCK细胞上发现了丝状病毒颗粒束以及杆状病毒颗粒。在感染乙型病毒株的MDCK细胞上观察到像绳子一样扭曲的丝状病毒颗粒。
