Jusa E R, Inaba Y
Department of Veterinary Epizootiology, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
J Basic Microbiol. 1995;35(5):315-8. doi: 10.1002/jobm.3620350506.
Kasba virus grown in BHK21-WI2 cells was tested for hemagglutination (HA) with erythrocytes of a variety of species at 4 degrees C, 25 degrees C and 37 degrees C. HA was observed at all temperatures with cattle, sheep and goat but not with swine, chicken, and goose erythrocytes. The HA was dependent on not only the NaCl concentration but also the pH of the diluent. The HA titer significantly improved by increasing the NaCl molarity to 0.6 M and standardizing pH to 7.5. The HA titer was 16- or 32-fold higher in 0.4 M or 0.6 M solution than in 0.2 M solution of not only NaCl but also several other salts. The HA reaction was inhibited by specific antibody.
在BHK21-WI2细胞中培养的卡斯巴病毒，在4℃、25℃和37℃下用多种物种的红细胞进行血凝试验（HA）。在所有温度下，牛、绵羊和山羊的红细胞均观察到血凝现象，而猪、鸡和鹅的红细胞则未观察到。血凝不仅取决于氯化钠浓度，还取决于稀释剂的pH值。通过将氯化钠摩尔浓度提高到0.6M并将pH值标准化为7.5，血凝效价显著提高。在0.4M或0.6M溶液中，血凝效价比在0.2M溶液中（不仅是氯化钠，还有其他几种盐）高16倍或32倍。血凝反应被特异性抗体抑制。
