Baba S S
Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria.
Arch Virol. 1993;128(1-2):155-61. doi: 10.1007/BF01309796.
The haemagglutinating (HA) properties of the Nigerian strain of Wesselsbron virus have been investigated using erythrocytes from a wide range of animals. The results showed that Wesselsbron virus possesses HA activity when extracted using the sucrose and acetone method. The erythrocytes of goose, horse, donkey, pig, cattle, sheep, goat, monkey, man, rabbit, rat, guinea pig and chicken were agglutinated by Wesselsbron virus at different pH values (5.75-7.0) and temperatures of 4 degrees C, room (25 +/- 2 degrees C) and 37 degrees C. The ability to haemagglutinate fell as pH increased, but the effect of incubation at different temperature was not marked. However, under the conditions of the experiment HA pattern was clearest at 37 degrees C. High HA titres (> or = 1:16) were consistently obtained using goose, horse, donkey and human erythrocytes at different temperatures.
利用多种动物的红细胞，对尼日利亚韦塞尔斯布朗病毒株的血凝（HA）特性进行了研究。结果表明，采用蔗糖和丙酮法提取时，韦塞尔斯布朗病毒具有HA活性。在不同pH值（5.75 - 7.0）以及4℃、室温（25±2℃）和37℃的温度条件下，韦塞尔斯布朗病毒能凝集鹅、马、驴、猪、牛、羊、山羊、猴、人、兔、大鼠、豚鼠和鸡的红细胞。随着pH值升高，血凝能力下降，但不同温度孵育的影响不显著。然而，在实验条件下，37℃时HA模式最为清晰。在不同温度下，使用鹅、马、驴和人红细胞均能持续获得高HA滴度（≥1:16）。
