Neurath A R, Strick N
J Gen Virol. 1979 Mar;42(3):645-9. doi: 10.1099/0022-1317-42-3-645.
Immunoprecipitates obtained by reacting Dane particle cores with human antibodies to hepatitis B core antigen (HBcAg) were chromatographed on columns of Sepharose 4B CL using 3 M-NaSCN as eluant. An antigen having the size and immunological specificity of monomeric e-antigen (HBeAg) was separated from HBcAg by this method. Antisera from animals immunized with HBcAg or HBeAg reacted not only with the antigen used for immunization but also with HBeAg and HBcAg, respectively. This indicates that HBeAg determinants are associated with the core of Dane particles.
将Dane颗粒核心与抗乙型肝炎核心抗原（HBcAg）的人抗体反应得到的免疫沉淀物，以3M硫氰酸钠作为洗脱剂，在交联琼脂糖4B柱上进行层析。通过这种方法从HBcAg中分离出了具有单体e抗原（HBeAg）大小和免疫特异性的一种抗原。用HBcAg或HBeAg免疫动物获得的抗血清，不仅与用于免疫的抗原发生反应，而且分别与HBeAg和HBcAg发生反应。这表明HBeAg决定簇与Dane颗粒的核心相关。
