慢性乙型肝炎病毒感染患者中与 Dane 颗粒相关的乙型肝炎 e 抗原和乙型肝炎 e 抗体

Dane particle-associated hepatitis B e antigen in patients with chronic hepatitis B virus infection and hepatitis B e antibody.

作者信息

Raimondo G, Recchia S, Lavarini C, Crivelli O, Rizzetto M

出版信息

Hepatology. 1982 Jul-Aug;2(4):449-54. doi: 10.1002/hep.1840020410.

DOI:10.1002/hep.1840020410

Abstract

A commercial radioimmunoassay was adapted to detect serum Dane particle-associated HBeAg in patients whose sera contained homologous antibody. HBeAg was released from Dane particles with guanidine HCl. Dane particles were separated from anti-HBe by gel-filtration (Sepharose 4B) and ultracentrifugation of the eluate. Dane particle-HBeAg was tested in 45 HBsAg carriers with anti-HBe and was present in 8 (18%) carriers, all of whom had chronic liver disease. By contrast, HBeAg was not found in 10 carriers with normal liver histology. Serum or liver HBcAg was found in 6 of 8 patients with Dane particle-HBeAg. None of the carriers without Dane particle-HBeAg had other markers of hepatitis B virion synthesis. We conclude that Dane particle-HBeAg provides a sensitive index of active hepatitis B virus replication, a guide to the presence of chronic hepatitis in HBsAg carriers with anti-HBe, and a noninvasive method to follow infection in these patients.

摘要

采用一种商业放射免疫分析法，以检测血清中含有同源抗体的患者血清中与丹氏颗粒相关的HBeAg。用盐酸胍从丹氏颗粒中释放HBeAg。通过凝胶过滤（琼脂糖4B）和对洗脱液进行超速离心，将丹氏颗粒与抗-HBe分离。对45例携带抗-HBe的HBsAg携带者检测丹氏颗粒-HBeAg，其中8例（18%）携带者呈阳性，所有这些携带者均患有慢性肝病。相比之下，10例肝组织学正常的携带者未检测到HBeAg。在8例丹氏颗粒-HBeAg阳性患者中，6例血清或肝组织中检测到HBcAg。没有丹氏颗粒-HBeAg的携带者均无其他乙肝病毒体合成标志物。我们得出结论，丹氏颗粒-HBeAg是乙肝病毒活跃复制的敏感指标，是抗-HBe阳性HBsAg携带者中慢性肝炎存在情况的指导指标，也是跟踪这些患者感染情况的非侵入性方法。