Cantiello H F
Renal Unit, Massachusetts General Hospital, Charlestown, USA.
Kidney Int. 1995 Oct;48(4):970-84. doi: 10.1038/ki.1995.379.
The regulatory role of actin filament organization on epithelial Na+ channel activity is reviewed in this report. The actin cytoskeleton, consisting of actin filaments and associated actin-binding proteins, is essential to various cellular events including the maintenance of cell shape, the onset of cell motility, and the distribution and stability of integral membrane proteins. Functional interactions between the actin cytoskeleton and specific membrane transport proteins are, however, not as well understood. Recent studies from our laboratory have determined that dynamic changes in the actin cytoskeletal organization may represent a novel signaling mechanism in the regulation of ion transport in epithelia. This report summarizes work conducted in our laboratory leading to an understanding of the molecular steps associated with the regulatory role of the actin-based cytoskeleton on epithelial Na+ channel function. The basis of this interaction lies on the regulation by actin-binding proteins and adjacent structures, of actin filament organization which in turn, modulates ion channel activity. The scope of this interaction may extend to such relevant cellular events as the vasopressin response in the kidney.
本报告综述了肌动蛋白丝组织对上皮钠通道活性的调节作用。由肌动蛋白丝和相关肌动蛋白结合蛋白组成的肌动蛋白细胞骨架，对于包括维持细胞形状、细胞运动的起始以及整合膜蛋白的分布和稳定性在内的各种细胞事件至关重要。然而，肌动蛋白细胞骨架与特定膜转运蛋白之间的功能相互作用尚未得到很好的理解。我们实验室最近的研究已经确定，肌动蛋白细胞骨架组织的动态变化可能代表了上皮细胞中离子转运调节的一种新的信号机制。本报告总结了我们实验室开展的工作，这些工作使我们了解了与基于肌动蛋白的细胞骨架对上皮钠通道功能的调节作用相关的分子步骤。这种相互作用的基础在于肌动蛋白结合蛋白和相邻结构对肌动蛋白丝组织的调节，而肌动蛋白丝组织反过来又调节离子通道活性。这种相互作用的范围可能扩展到诸如肾脏中抗利尿激素反应等相关细胞事件。
