Sheldon W G, Warbritton A R, Bucci T J, Turturro A
Pathology Associates, Inc., Jefferson, AR 72079, USA.
Lab Anim Sci. 1995 Oct;45(5):508-18.
We allocated 110 DBA/2NNia mice of either sex to one of two feeding regimens: ad libitum (AL) or food restriction (FR) to 60% of the amount consumed by the AL group. The mice were examined at 3, 6, 9, 12, 18, and 24 months (at 3 months, only AL mice were examined). During the remaining periods approximately equal numbers (n = 10) of mice of both sexes and diet groups were examined. Peripheral anterior synechia was the first glaucoma-associated lesion observed and was present in 8 of 10 AL female mice, 6 of 10 AL males, and 1 FR male at 6 months. At 9 months peripheral anterior synechia was present in all AL females and was accompanied by depletion of retinal ganglion cells and degeneration of the optic nerves and optic tracts. Ninety percent of the eyes in the AL males also had peripheral anterior synechia at 9 months, but ganglion cell depletion and optic nerve degeneration were not observed as frequently. Neovascular membranes in the iridocorneal angle, a component of peripheral anterior synechia, were first observed at 9 months in approximately 55% of the globes of the AL mice and 5% of the FR mice. This was a major difference in the microscopic features of synechia between the diet groups and resulted in increased severity of synechia in the AL mice compared with their FR cohorts. Degeneration of the optic nerves and tracts was characterized by atrophy, astrogliosis, increase in cellularity, fragmentation of axons, and loss of myelin. Glaucoma in the FR mice of both sexes was less severe than in their AL counterparts. The most severely affected were AL females, followed by FR females, AL males, and FR males. Food restriction reduced the incidence and severity of the ocular lesions in females at all periods. The primary benefit of FR in males occurred during the 6- and 9-month periods when the incidence and severity of the glaucoma-related lesions were reduced; in the succeeding months the major benefit was minimal reduction of the severity of the lesions.
我们将110只不同性别的DBA/2NNia小鼠分配到两种喂养方案之一：自由采食（AL）或食物限制（FR），即给予FR组小鼠的食物量为AL组小鼠消耗量的60%。在3、6、9、12、18和24个月时对小鼠进行检查（3个月时，仅检查AL组小鼠）。在其余时间段，对两个饮食组中大致相等数量（n = 10）的不同性别的小鼠进行检查。周边前粘连是观察到的首个与青光眼相关的病变，在6个月时，10只AL组雌性小鼠中有8只、10只AL组雄性小鼠中有6只以及1只FR组雄性小鼠出现了该病变。9个月时，所有AL组雌性小鼠均出现周边前粘连，并伴有视网膜神经节细胞减少以及视神经和视束变性。9个月时，90%的AL组雄性小鼠眼睛也出现了周边前粘连，但神经节细胞减少和视神经变性的情况不如雌性小鼠频繁。虹膜角膜角新生血管膜是周边前粘连的一个组成部分，在9个月时首次在约55%的AL组小鼠眼球和5%的FR组小鼠眼球中观察到。这是两个饮食组之间粘连微观特征的一个主要差异，导致AL组小鼠的粘连严重程度高于其FR组同窝小鼠。视神经和视束变性的特征为萎缩、星形胶质细胞增生、细胞增多、轴突断裂和髓鞘丢失。两个性别的FR组小鼠的青光眼病情均不如其AL组同窝小鼠严重。受影响最严重的是AL组雌性小鼠，其次是FR组雌性小鼠、AL组雄性小鼠和FR组雄性小鼠。食物限制在各个时期均降低了雌性小鼠眼部病变的发生率和严重程度。FR对雄性小鼠的主要益处出现在6至9个月期间，此时与青光眼相关病变的发生率和严重程度均有所降低；在随后的几个月中，主要益处是病变严重程度略有降低。
