Giorgini D, Taha M K
Unité des Neisseria, Institut Pasteur, Paris, France.
Mol Cell Probes. 1995 Oct;9(5):297-306. doi: 10.1016/s0890-8508(95)91540-0.
A new molecular typing method for identification and characterization of Neisseria meningitidis is reported. Chromosomal DNA from 20 well-documented meningococcal strains of serogroup A originating from France, Central African Republic, Sudan and Burkina Faso were amplified using the polymerase chain reaction. Primers designed in this study were located in the pilA/pilB locus which has been shown to be conserved in the genus Neisseria. The amplified fragments were subjected to restriction endonuclease analysis using three different enzymes, and the restriction endonuclease patterns obtained were compared. Clonal isolates clustered together in distinct restriction endonuclease patterns which are described in this study and coincided with electrotypes as determined by multi-locus enzyme electrophoresis. This DNA-based typing system for meningococci may be useful for epidemiological studies.
报道了一种用于鉴定和表征脑膜炎奈瑟菌的新分子分型方法。使用聚合酶链反应扩增了来自法国、中非共和国、苏丹和布基纳法索的20株记录完备的A群脑膜炎球菌菌株的染色体DNA。本研究设计的引物位于pilA/pilB基因座，该基因座已被证明在奈瑟菌属中是保守的。将扩增片段用三种不同的酶进行限制性内切酶分析，并比较所得的限制性内切酶图谱。克隆分离株以不同的限制性内切酶图谱聚集在一起，本研究对此进行了描述，并且与多位点酶电泳确定的电型一致。这种基于DNA的脑膜炎球菌分型系统可能对流行病学研究有用。
