Auriol J, Guesdon J L, Guibourdenche M, Riou J Y
Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.
FEMS Immunol Med Microbiol. 1995 Feb;10(3-4):219-26. doi: 10.1111/j.1574-695X.1995.tb00036.x.
We studied 35 strains of Neisseria meningitidis serogroup A from different locations (France, Central African Republic, Sudan and Burkina Faso) using both ribotyping and a polymerase chain reaction (PCR). A non-radioactive probe label was used for ribotyping; detection consisted of an immunoenzymatic procedure using a bispecific antibody. The PCR was designed to amplify the 16S-23S rDNA internal transcribed spacer. These techniques were compared with other markers. The strains were identified as belonging to three clones (I, III-1, IV) by multilocus enzyme electrophoresis (MEE) and to three subtypes by serological methods. Ribotyping identified five groups and PCR identified four groups. Ribotyping gave more diversity between strains than either MEE or sero/subtyping, but confirmed the epidemiological data provided by the combination of these two techniques. The PCR provided a simple and convenient one-step procedure for the differentiation of strains of serogroup A.
我们使用核糖体分型和聚合酶链反应（PCR）对来自不同地点（法国、中非共和国、苏丹和布基纳法索）的35株A群脑膜炎奈瑟菌进行了研究。核糖体分型使用了非放射性探针标记；检测采用双特异性抗体的免疫酶法。PCR旨在扩增16S - 23S rDNA内部转录间隔区。将这些技术与其他标记进行了比较。通过多位点酶电泳（MEE）将这些菌株鉴定为属于三个克隆（I、III - 1、IV），通过血清学方法鉴定为三个亚型。核糖体分型鉴定出五组，PCR鉴定出四组。核糖体分型显示菌株间的多样性比MEE或血清/亚型分型更多，但证实了这两种技术联合提供的流行病学数据。PCR为A群菌株的鉴别提供了一种简单便捷的一步法。
