Ji Q, Chen Y
Department of Biology, Nanjing Normal University, China.
Mutat Res. 1996 Jan 16;359(1):1-6. doi: 10.1016/s0165-1161(96)90003-5.
The possible mutagenicity of the water-soluble contents of cigarette smoke (WSCS) was evaluated by using the Vicia faba root tip micronucleus test. The results showed significant changes in micronucleus frequency which were caused by each different concentration of WSCS. This indicates that the Vicia faba root tip micronucleus test might be used as one kind of mutagenic detection method for cigarette smoke. A comparative evaluation on the mutagenicity of 10 brands of cigarettes was carried out. Results confirmed that various degrees of mutagenicity were found for all of the brand cigarettes, among them, Huaihai was the highest, while Camellia was the lowest. The micronucleus frequencies were reduced by adding tea polyphenol, nicotinamide adenine, vitamin C and sodium selenite to the WSCS. The results suggest that these added substances might reduce the genetic injury induced by cigarette smoke.
利用蚕豆根尖微核试验评估了香烟烟雾水溶性成分（WSCS）的潜在致突变性。结果显示，不同浓度的WSCS均导致微核频率发生显著变化。这表明蚕豆根尖微核试验可作为一种检测香烟烟雾致突变性的方法。对10个品牌香烟的致突变性进行了比较评估。结果证实，所有品牌香烟均有不同程度的致突变性，其中淮海牌香烟的致突变性最高，茶花牌香烟的致突变性最低。向WSCS中添加茶多酚、烟酰胺腺嘌呤、维生素C和亚硒酸钠后，微核频率降低。结果表明，这些添加物质可能会减轻香烟烟雾引起的遗传损伤。
