Yadav J S, Kaushik V K
Human Genetics Unit, Kurukshetra University, India.
Mutat Res. 1996 Jan 16;359(1):25-9. doi: 10.1016/s0165-1161(96)90006-0.
The genotoxic effects of an average concentration of 41.7 mg/m3 of SO2 exposure on 42 workers of a fertilizer factory were investigated. Mitotic index (MI), chromosomal aberrations (CAs), sister-chromatid exchanges (SCEs) and satellite associations (SA) were observed. In SO2-exposed workers, a higher mitotic index (7.09) was recorded in comparison to controls (4.34). The MI, however, declined with duration of exposure. Satellite associations showed a two-fold increase (17.1) as compared to controls (8.11). Among chromosomes, D-G group associations were the highest (7.43%), while 3D type associations were the lowest (0.4%). There was a significant difference (p < 0.05) in the mean frequency of CAs per cell in the exposed workers (3.262%) and the controls (0.833%). The mean frequency of SCEs per cell increased from 3.32 +/- 0.1 in controls to 7.72 +/- 0.19 in the exposed group. The difference was significant (p < 0.05). In smokers, alcoholics and smoker-alcoholics, the frequency of CAs and SCEs per cell was significantly higher than the non-smokers and non-alcoholics, both in the controls and the SO2-exposed workers and showed a correlation with the duration of exposure. SO2 is therefore a clastogenic and genotoxic agent for which necessary precautions must be taken.
对一家化肥厂42名工人暴露于平均浓度为41.7毫克/立方米的二氧化硫环境中的遗传毒性作用进行了研究。观察了有丝分裂指数（MI）、染色体畸变（CA）、姐妹染色单体交换（SCE）和卫星联合（SA）。在接触二氧化硫的工人中，与对照组（4.34）相比，记录到更高的有丝分裂指数（7.09）。然而，有丝分裂指数随暴露时间的延长而下降。卫星联合与对照组（8.11）相比增加了两倍（17.1）。在染色体中，D - G组联合最高（7.43%），而3D型联合最低（0.4%）。暴露工人（3.262%）和对照组（0.833%）每细胞染色体畸变的平均频率存在显著差异（p < 0.05）。每细胞姐妹染色单体交换的平均频率从对照组的3.32±0.1增加到暴露组的7.72±0.19。差异显著（p < 0.05）。在吸烟者、酗酒者和吸烟酗酒者中，对照组和接触二氧化硫的工人每细胞染色体畸变和姐妹染色单体交换的频率均显著高于非吸烟者和非酗酒者，且与暴露时间相关。因此，二氧化硫是一种断裂剂和遗传毒性剂，必须采取必要的预防措施。
