Asikainen M, Deboer T, Porkka-Heiskanen T, Stenberg D, Tobler I
Institute of Biomedicine, University of Helsinki, Finland.
Neurosci Lett. 1995 Sep 22;198(1):21-4. doi: 10.1016/0304-3940(95)11953-t.
Djungarian hamsters well adapted to a short photoperiod were subjected to 4 h of total sleep deprivation (SD) by gentle handling. Tissue concentrations of monoamines and of their metabolites were measured from several brain areas using HPLC with electrochemical detection. The 5-hydroxyindoleacetic acid/5-hydroxytryptamine (5-HIAA/5-HT) ratio was significantly increased after SD in the hippocampus, hypothalamus and brain stem, indicating increased serotonin (5-HT) turnover in those areas, while no changes were found in the frontal cortex and olfactory bulb. Dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) concentrations were elevated in the hypothalamus, while the noradrenaline concentrations did not change in any of the measured areas. We conclude that a short SD, which has been shown to elevate EEG slow-wave activity during recovery sleep, specifically increases 5-HT turnover in the brain.
将适应短光照周期的西伯利亚仓鼠通过轻柔处理进行4小时的完全睡眠剥夺（SD）。使用带电化学检测的高效液相色谱法（HPLC）测量几个脑区中单胺及其代谢物的组织浓度。睡眠剥夺后，海马体、下丘脑和脑干中的5-羟吲哚乙酸/5-羟色胺（5-HIAA/5-HT）比值显著升高，表明这些区域中5-羟色胺（5-HT）的周转率增加，而额叶皮质和嗅球未发现变化。下丘脑多巴胺和3,4-二羟基苯乙酸（DOPAC）浓度升高，而在所测量的任何区域中去甲肾上腺素浓度均未改变。我们得出结论，短时间睡眠剥夺已被证明可提高恢复睡眠期间的脑电图慢波活动，它会特异性增加大脑中5-HT的周转率。
