Eberle K E, Nguyen V T, Freistadt M S
Department of Microbiology, Immunology and Parasitology, Louisiana State University Medical Center, New Orleans, USA.
Arch Virol. 1995;140(12):2135-50. doi: 10.1007/BF01323236.
To investigate the molecular mediators of poliovirus tissue tropism, the correlation between poliovirus replication and poliovirus receptor expression was examined in a primary human tissue system. Earlier work [M. Freistadt, H. Fleit, and E. Wimmer, Virology 195: 798-803 (1993)] showed that the cellular receptor for poliovirus is present in 87% of primary human monocytes and that peripheral blood mononuclear cells support poliovirus replication. In the current work, monocytes, obtained by adherence or by a novel negative selection procedure using specific monoclonal antibodies to lymphocyte surface antigens, supported poliovirus replication. However, total virus yield was low and infectious centers assays revealed that a minority (6%) of monocytes become productively infected. Viral yield from monocytes was lower than from the heterogeneous mononuclear cells; however, when uninfected lymphocytes were added back to infected monocytes, the higher viral yield was restored. The purity of the cells did not significantly affect the number of cells infected. These results suggest that more poliovirus is produced per cell from activated rather than unactivated monocytes. Furthermore, poliovirus replication in monocytes may reflect genuine in vivo replication and comprise a system in which to determine molecular mediators of poliovirus tissue tropism.
为了研究脊髓灰质炎病毒组织嗜性的分子介质，在原代人体组织系统中检测了脊髓灰质炎病毒复制与脊髓灰质炎病毒受体表达之间的相关性。早期研究[M. 弗赖施塔特、H. 弗莱特和E. 维默，《病毒学》195: 798 - 803(1993)]表明，87%的原代人单核细胞中存在脊髓灰质炎病毒的细胞受体，并且外周血单核细胞支持脊髓灰质炎病毒复制。在当前研究中，通过贴壁或使用针对淋巴细胞表面抗原的特异性单克隆抗体的新型阴性选择程序获得的单核细胞支持脊髓灰质炎病毒复制。然而，病毒总产量较低，感染中心检测显示少数(6%)单核细胞发生有效感染。单核细胞产生的病毒量低于异质性单核细胞；然而，当将未感染的淋巴细胞重新添加到感染的单核细胞中时，病毒产量恢复到较高水平。细胞纯度对感染细胞数量没有显著影响。这些结果表明，活化的单核细胞比未活化的单核细胞每个细胞产生更多的脊髓灰质炎病毒。此外，脊髓灰质炎病毒在单核细胞中的复制可能反映了体内真实的复制情况，并构成了一个用于确定脊髓灰质炎病毒组织嗜性分子介质的系统。
