Anagnostopoulos I, Hummel M, Stein H
Institute of Pathology, Klinikum Benjamin Franklin, Free University Berlin, Germany.
Leuk Lymphoma. 1995 Sep;19(1-2):1-12. doi: 10.3109/10428199509059657.
Two-hundred and four cases of peripheral T cell lymphoma (PTCL) occurring in Europeans without any sign of HIV-infection were investigated for their association with an Epstein-Barr virus (EBV) infection. Polymerase chain reaction (PCR) was applied for EBV-DNA detection, in situ hybridisation (ISH) for the cellular localization of EBV-encoded small nuclear RNA (EBER) and immediate early gene expression (BHLF) and immunohistology (IH) for the detection of EBV-encoded latent membrane protein 1 (LMP1) and EBV nuclear antigen 2 (EBNA2) expression. PCR and EBER-ISH produced congruent results in almost all cases with amplifiable DNA, leading to the finding of an overall frequency of EBV presence in 87/204 (42.6%) of the PTCL cases. Through EBER-ISH, the virus was identified to be exclusively present in small and blastic bystander lymphocytes in 29 cases, whilst an additional infection of neoplastic T cells was observed in the remaining 58 EBV-positive cases. The entity presenting with the most frequent EBV infection of tumour cells was that of angioimmunoblastic type PTCL, whilst the primary cutaneous PTCLs only seldom harbored the virus. Forty-eight of the EBV-positive TCLs showed an infection of a small proportion (1-20%) of the tumour cell population, whilst another ten cases, belonging to the pleomorphic TCL (PMTCL) group, displayed an infection of several to almost all neoplastic T cells (20-100%). Additional lytic EBV-infected cells could be detected in four cases by BHLF-ISH. LMP1 expression was present in a small proportion of the neoplastic T cells in 24 of the 58 cases with tumour cell infection, whilst an EBNA2 expression was detectable only in one case. Some non-malignant EBV-infected B-immunoblasts and Hodgkin/Reed Sternberg-like cells also expressed LMP1 in several cases. Our data imply a role of EBV in the pathogenesis of only a few PMTCL cases with predominant tumour cell infection, whilst the pathogenic significance of an EBV infection in the other PTCLs remains unclear due to the usually partial infection of the neoplastic cell component.
对204例无任何HIV感染迹象的欧洲人外周T细胞淋巴瘤（PTCL）病例进行了研究，以探讨其与爱泼斯坦-巴尔病毒（EBV）感染的相关性。采用聚合酶链反应（PCR）检测EBV-DNA，原位杂交（ISH）检测EBV编码的小核RNA（EBER）和即刻早期基因表达（BHLF）的细胞定位，免疫组织化学（IH）检测EBV编码的潜伏膜蛋白1（LMP1）和EBV核抗原2（EBNA2）的表达。在几乎所有可扩增DNA的病例中，PCR和EBER-ISH结果一致，结果显示PTCL病例中EBV存在的总体频率为87/204（42.6%）。通过EBER-ISH，在29例病例中，病毒仅存在于小的母细胞样旁观者淋巴细胞中，而在其余58例EBV阳性病例中观察到肿瘤性T细胞的额外感染。肿瘤细胞EBV感染最常见的实体是血管免疫母细胞型PTCL，而原发性皮肤PTCL很少携带该病毒。48例EBV阳性TCL显示肿瘤细胞群体中有一小部分（1%-20%）受到感染，而另外10例属于多形性TCL（PMTCL）组，显示几个至几乎所有肿瘤性T细胞（20%-100%）受到感染。通过BHLF-ISH在4例病例中检测到额外的EBV裂解感染细胞。在58例肿瘤细胞感染的病例中，24例肿瘤性T细胞中有一小部分表达LMP1，而仅在1例病例中可检测到EBNA2表达。在一些病例中，一些非恶性EBV感染的B免疫母细胞和霍奇金/里德-斯腾伯格样细胞也表达LMP1。我们的数据表明，EBV仅在少数以肿瘤细胞感染为主的PMTCL病例的发病机制中起作用，而由于肿瘤细胞成分通常为部分感染，EBV感染在其他PTCL中的致病意义仍不清楚。
