Anagnostopoulos I, Hummel M, Tiemann M, Korbjuhn P, Parwaresch M R, Stein H
Institute of Pathology, Klinikum Steglitz, Free University of Berlin, Germany.
Histopathology. 1994 Oct;25(4):331-7. doi: 10.1111/j.1365-2559.1994.tb01351.x.
This study deals with the investigation of the biological significance of an Epstein-Barr virus (EBV) infection in lymphoepithelioid cell lymphoma. A selection of EBV-detection techniques was applied to 15 cases, including polymerase chain reaction (PCR) for the detection of EBV-DNA, in situ hybridization (ISH) for the cellular localization of EBV-encoded small nuclear (EBER 1 and EBER 2) and immediate-early (BHLF) RNAs, and immunohistology for the detection of EBV-encoded latent membrane protein (LMP) expression. PCR and EBER-ISH produced congruent results in those cases with amplifiable DNA, leading to an EBV presence in 11/15 lymphoepithelioid cell lymphoma cases (73%). EBER-ISH combined with immunohistology localized the virus predominantly in several B immunoblasts and small B lymphocytes in eight of the EBV-positive cases, five of which also contained single infected lymphocytes expressing T-cell characteristic antigens. LMP was detected using immunohistology in only a proportion of immunoblasts in four of these cases. The remaining three EBV-positive lymphoepithelioid cell lymphoma cases contained only single EBER-positive small B lymphocytes without LMP expression. No case contained BHLF-RNA expressing cells. These data imply that, although latently EBV-infected cells are frequently present in lymphoepithelioid cell lymphoma cases, the virus is probably not directly involved in the pathogenesis of this entity.
本研究旨在调查爱泼斯坦-巴尔病毒（EBV）感染在淋巴上皮样细胞淋巴瘤中的生物学意义。对15例病例应用了一系列EBV检测技术，包括用于检测EBV-DNA的聚合酶链反应（PCR）、用于EBV编码的小核RNA（EBER 1和EBER 2）及立即早期RNA（BHLF）细胞定位的原位杂交（ISH），以及用于检测EBV编码的潜伏膜蛋白（LMP）表达的免疫组织学。在那些DNA可扩增的病例中，PCR和EBER-ISH产生了一致的结果，15例淋巴上皮样细胞淋巴瘤病例中有11例（73%）检测到EBV存在。在8例EBV阳性病例中，EBER-ISH联合免疫组织学显示病毒主要定位于几个B免疫母细胞和小B淋巴细胞，其中5例还含有表达T细胞特征性抗原的单个感染淋巴细胞。在其中4例病例中，仅在一部分免疫母细胞中通过免疫组织学检测到LMP。其余3例EBV阳性淋巴上皮样细胞淋巴瘤病例仅含有单个EBER阳性的小B淋巴细胞，无LMP表达。没有病例含有表达BHLF-RNA的细胞。这些数据表明，虽然在淋巴上皮样细胞淋巴瘤病例中经常存在潜伏性EBV感染的细胞，但该病毒可能并不直接参与该实体的发病机制。
