Karal'nik B V, Tsarevskiĭ Iu P, Shamardin V A, Shmuter M F
Zh Mikrobiol Epidemiol Immunobiol. 1977 Feb(2):120-5.
Quantitative methods were applied to the study of the interaction of albumins, fraction I of Plague bacilli, diphtheria toxoid fractions differing by mol wt, flagellin of typhoid bacilli, 19S- and 7S-fractions of normal human, cholera horse, and paratyphoid B donkey sera with erythrocytes, fixed by 10 different methods. Fixation with acetaldehyde proved to be optimal for the binding of all the proteins, including flagellin, but the latter sensitized erythrocytes formalinized after Vainbach better. The significance of the method of erythrocyte fixation and of the nature of sensitin in the process of the erythrocyte loading without any utilization of the conjugating agents was demonstrated.
运用定量方法研究了白蛋白、鼠疫杆菌I组分、不同分子量的白喉类毒素组分、伤寒杆菌鞭毛蛋白、正常人、霍乱马和副伤寒B驴血清的19S和7S组分与通过10种不同方法固定的红细胞之间的相互作用。结果表明，用乙醛固定对于包括鞭毛蛋白在内的所有蛋白质的结合最为理想，但鞭毛蛋白对经万巴赫法甲醛固定后的红细胞致敏效果更好。在不使用偶联剂的红细胞负载过程中，证明了红细胞固定方法和致敏素性质的重要性。
