Karal'nik B V, Melikhova R B, Shamardin V A, Tsarevskiĭ Iu P
Zh Mikrobiol Epidemiol Immunobiol. 1978 Sep(9):91-7.
The effect of some methods of preliminary treatment of erythrocytes on the PHAT depended on the sensitin náture and the method of erythrocyte load. In case of erythrocyte load with nonprotein and immunoglobulin sensitins without any conjugating agents the simulating effect of heating and periodate treatment was caused not by increase of stable sensitin binding, but by the reduction of physico-chemical resistance of erythrocytes. This effect of erythrocyte treatment permitted to increase the sensitivity of the antibodies and antigens determination. In loading the erythrocytes with the aid of conjugating agents and in sensitization with protein antigens after Boyden no stimuating effect of the treatment was noted.
某些红细胞预处理方法对被动血凝试验（PHAT）的影响取决于致敏素的性质和红细胞负载的方法。在用非蛋白质和免疫球蛋白致敏素负载红细胞且无任何结合剂的情况下，加热和过碘酸盐处理的模拟效应并非由稳定致敏素结合的增加引起，而是由红细胞物理化学抗性的降低所致。这种红细胞处理效应使得抗体和抗原测定的灵敏度得以提高。在用结合剂负载红细胞以及在博伊登法后用蛋白质抗原致敏时，未观察到处理的刺激效应。
