Slomka M J, Ashley R L, Cowan F M, Cross A, Brown D W
Enteric and Respiratory Virus Laboratory, Central Public Health Laboratory, London, UK.
J Virol Methods. 1995 Sep;55(1):27-35. doi: 10.1016/0166-0934(95)00042-s.
Monoclonal antibody blocking radioimmunoassays (MAb block RIAs) which detect specific humoral responses to each of the two human herpes simplex virus (HSV) types are described. RIAs were compared with Western blot assay (WBA) in a blind study of 64 sera obtained from clinically well-documented cases of genital herpes. WBA and MAb block RIA each detected HSV-1 antibodies in 16/17 (94%) sera from confirmed HSV-1 genital infections (first episodes and recurrent infections). Detection of HSV-2 antibody in 21 sera from HSV-2 first episodes was more effective by WBA which identified homologous antibody in 19 (96%), whereas MAb block RIA detected HSV-2 antibody in 16 (76%). HSV-2 antibody was detected in 24/25 (96%) sera from recurrent HSV-2 infections by WBA and by MAb block RIA, the highest degree of concordance for both methods. In addition, the MAb block RIA may be more effective in detecting the presence of HSV-1 antibody in sera from recurrent HSV-2 cases. Prevalence of HSV-1 and HSV-2 antibody was measured by the MAb block RIAs in 3 UK human study populations which consisted of 100 children/young adolescents, 104 adult blood donors and 80 genito-urinary medicine clinic attenders.
本文描述了单克隆抗体阻断放射免疫测定法（MAb block RIAs），该方法可检测针对两种人类单纯疱疹病毒（HSV）各型的特异性体液免疫反应。在一项对64份血清的盲法研究中，将放射免疫测定法与蛋白质印迹法（WBA）进行了比较，这些血清取自临床记录完整的生殖器疱疹病例。在确诊为HSV-1生殖器感染（初发和复发感染）的17份血清中，有16份（94%）通过WBA和MAb block RIA检测到HSV-1抗体。在21份HSV-2初发病例的血清中，WBA检测HSV-2抗体的效果更佳，能识别出19份（96%）中的同源抗体，而MAb block RIA仅在16份（76%）中检测到HSV-2抗体。在25份HSV-2复发感染的血清中，WBA和MAb block RIA均在24份（96%）中检测到HSV-2抗体，这是两种方法一致性最高的情况。此外，MAb block RIA在检测HSV-2复发病例血清中的HSV-1抗体时可能更有效。通过MAb block RIAs对3个英国人群进行了HSV-1和HSV-2抗体的患病率检测，这3个群体分别为100名儿童/青少年、104名成年献血者和80名泌尿生殖医学门诊就诊者。
