利用重组gG2、蛋白质印迹法和gG2抑制法检测非洲血清中2型单纯疱疹病毒特异性免疫球蛋白G抗体。
Detection of herpes simplex virus type 2-specific immunoglobulin G antibodies in African sera by using recombinant gG2, Western blotting, and gG2 inhibition.
作者信息
Hogrefe Wayne, Su Xin, Song James, Ashley Rhoda, Kong Lilly
机构信息
Focus Technologies, 5785 Corporate Avenue, Cypress, CA 90630, USA.
出版信息
J Clin Microbiol. 2002 Oct;40(10):3635-40. doi: 10.1128/JCM.40.10.3635-3640.2002.
Abstract
Sera (n = 781) from four African countries were used to determine the prevalence of herpes simplex virus type 2 (HSV-2) antibodies by using the HerpeSelect HSV-2 enzyme-linked immunosorbent assay (ELISA; Focus Technologies) and Western blotting (WB). Also, an HSV inhibition assay was developed to evaluate the discordant sample results between HerpesSelect and WB. The seroprevalence of HSV-2 ranged from 17% in the South African panel to nearly 70% in panels from Kenya, Uganda, and Zimbabwe. Overall, HerpeSelect was 100% sensitive and 88% specific compared to WB and 100% sensitive and 96% specific compared to the inhibition assay. There was 100% concordance among all three assays for samples from South Africa and Zimbabwe. The discordant results occurred in samples from Kenya and Uganda.
摘要
来自四个非洲国家的781份血清样本被用于通过使用HerpeSelect HSV - 2酶联免疫吸附测定法（ELISA；Focus Technologies公司）和蛋白质印迹法（WB）来确定2型单纯疱疹病毒（HSV - 2）抗体的流行率。此外，还开发了一种HSV抑制试验来评估HerpesSelect和WB之间结果不一致的样本。HSV - 2的血清流行率在南非样本组中为17%，而在肯尼亚、乌干达和津巴布韦的样本组中接近70%。总体而言，与WB相比，HerpeSelect的敏感性为100%，特异性为88%；与抑制试验相比，敏感性为100%，特异性为96%。来自南非和津巴布韦的样本在所有三种检测方法之间的一致性为100%。不一致的结果出现在来自肯尼亚和乌干达的样本中。
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