Nascimento Maria Claudia, Ferreira Suzete, Sabino Ester, Hamilton Ingrid, Parry John, Pannuti Claudio S, Mayaud Philippe
Clinical Research Unit, Department of Infectious & Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.
J Clin Microbiol. 2007 Jul;45(7):2309-11. doi: 10.1128/JCM.00144-07. Epub 2007 May 16.
A total of 586 serum samples were used to evaluate the performance of type-specific herpes simplex virus type 2 (HSV-2) commercial enzyme-linked immunosorbent assays (ELISAs) by using the monoclonal antibody-blocking enzyme immunoassay (MAb-EIA) and a clinicovirological panel as reference standards. The Kalon and HerpeSelect ELISAs had similar sensitivities (93.5% and 93.8% compared with the results obtained by MAb-EIA, respectively, and 100% for both ELISAs compared with the results obtained with a clinicovirological panel). The Kalon ELISA had a higher specificity (96.5% and 96.8% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively) than the HerpeSelect ELISA (86.9% and 94% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively). A higher cutoff significantly improved the specificity of the HerpeSelect ELISA.
共使用586份血清样本，以单克隆抗体阻断酶免疫测定法（MAb-EIA）和临床病毒学检测组合作为参考标准，评估2型单纯疱疹病毒（HSV-2）特异性商用酶联免疫吸附测定法（ELISA）的性能。Kalon ELISA和HerpeSelect ELISA的灵敏度相似（与MAb-EIA结果相比，分别为93.5%和93.8%；与临床病毒学检测组合结果相比，两种ELISA均为100%）。Kalon ELISA的特异性高于HerpeSelect ELISA（与MAb-EIA结果相比为96.5%，与临床病毒学检测组合结果相比为96.8%），而HerpeSelect ELISA与MAb-EIA结果相比为86.9%，与临床病毒学检测组合结果相比为94%。提高临界值可显著提高HerpeSelect ELISA的特异性。
