在巴西，采用单克隆抗体阻断酶免疫测定法和临床病毒学参考标准，评估HerpeSelect（Focus）和Kalon酶联免疫吸附测定法检测抗2型单纯疱疹病毒抗体的性能。
Performance of the HerpeSelect (Focus) and Kalon enzyme-linked immunosorbent assays for detection of antibodies against herpes simplex virus type 2 by use of monoclonal antibody-blocking enzyme immunoassay and clinicovirological reference standards in Brazil.
作者信息
Nascimento Maria Claudia, Ferreira Suzete, Sabino Ester, Hamilton Ingrid, Parry John, Pannuti Claudio S, Mayaud Philippe
机构信息
Clinical Research Unit, Department of Infectious & Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.
出版信息
J Clin Microbiol. 2007 Jul;45(7):2309-11. doi: 10.1128/JCM.00144-07. Epub 2007 May 16.
Abstract
A total of 586 serum samples were used to evaluate the performance of type-specific herpes simplex virus type 2 (HSV-2) commercial enzyme-linked immunosorbent assays (ELISAs) by using the monoclonal antibody-blocking enzyme immunoassay (MAb-EIA) and a clinicovirological panel as reference standards. The Kalon and HerpeSelect ELISAs had similar sensitivities (93.5% and 93.8% compared with the results obtained by MAb-EIA, respectively, and 100% for both ELISAs compared with the results obtained with a clinicovirological panel). The Kalon ELISA had a higher specificity (96.5% and 96.8% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively) than the HerpeSelect ELISA (86.9% and 94% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively). A higher cutoff significantly improved the specificity of the HerpeSelect ELISA.
摘要
共使用586份血清样本，以单克隆抗体阻断酶免疫测定法（MAb-EIA）和临床病毒学检测组合作为参考标准，评估2型单纯疱疹病毒（HSV-2）特异性商用酶联免疫吸附测定法（ELISA）的性能。Kalon ELISA和HerpeSelect ELISA的灵敏度相似（与MAb-EIA结果相比，分别为93.5%和93.8%；与临床病毒学检测组合结果相比，两种ELISA均为100%）。Kalon ELISA的特异性高于HerpeSelect ELISA（与MAb-EIA结果相比为96.5%，与临床病毒学检测组合结果相比为96.8%），而HerpeSelect ELISA与MAb-EIA结果相比为86.9%，与临床病毒学检测组合结果相比为94%。提高临界值可显著提高HerpeSelect ELISA的特异性。
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