Pond C M, Mattacks C A
Department of Biology, Open University, Milton Keynes, United Kingdom.
J Lipid Res. 1995 Oct;36(10):2219-31.
The functional relationships between lymphoid cells and the adipose tissue that surrounds lymph nodes were investigated in healthy adult guinea pigs. Lymphoid cells extracted from healthy adult guinea pigs were co-cultured for 48 h with adipose tissue explants from 18 sites defined by their anatomical relations to lymph nodes. Such explants from near a node suppressed lymphocyte proliferation stimulated with concanavalin A or lipopolysaccharide more than those from sites 5-10 mm from nodes. Inhibition was almost completely abolished by 500 microU insulin. The presence of lymphoid cells increased lipolysis (measured as glycerol release) in adipose tissue from all depots containing lymph nodes (i.e., except perirenal), especially in the presence of mitogens and with near-node samples from intermuscular and mesenteric depots. Inhibition of lymphocyte proliferation by adipose tissue was proportional to the additional lipolysis stimulated by the presence of lymphoid cells. For all depots except the mesenteric, glycerol release stimulated by lymphoid cells was inversely proportional to spontaneous lipolysis in adipose tissue cultured alone. These experiments demonstrate reciprocal interactions between lymphoid cells and adipose tissue, especially that around lymph nodes. The mediators of the action of adipose tissue on lymphoid cells probably include lipolytic products; mediators of the inverse effects are unknown.
在健康成年豚鼠中研究了淋巴细胞与淋巴结周围脂肪组织之间的功能关系。从健康成年豚鼠中提取的淋巴细胞与来自18个根据其与淋巴结的解剖关系定义的部位的脂肪组织外植体共培养48小时。来自淋巴结附近的此类外植体比距离淋巴结5-10毫米处的外植体更能抑制伴刀豆球蛋白A或脂多糖刺激的淋巴细胞增殖。500微单位胰岛素几乎完全消除了这种抑制作用。淋巴细胞的存在增加了所有含有淋巴结的脂肪库（即肾周除外）的脂肪组织中的脂解作用（以甘油释放量衡量），尤其是在有丝分裂原存在时以及来自肌肉间和肠系膜脂肪库的淋巴结附近样本中。脂肪组织对淋巴细胞增殖的抑制作用与淋巴细胞存在所刺激的额外脂解作用成正比。对于除肠系膜外的所有脂肪库，淋巴细胞刺激的甘油释放量与单独培养的脂肪组织中的自发脂解作用成反比。这些实验证明了淋巴细胞与脂肪组织之间的相互作用，尤其是淋巴结周围的脂肪组织。脂肪组织对淋巴细胞作用的介质可能包括脂解产物；反向作用的介质尚不清楚。
