Breese E J, Michie C A, Nicholls S W, Williams C B, Domizio P, Walker-Smith J A, MacDonald T T
Department of Paediatric Gastroenterology, St Bartholomew's Hospital, London, UK.
Aliment Pharmacol Ther. 1995 Oct;9(5):547-52. doi: 10.1111/j.1365-2036.1995.tb00419.x.
Recent studies have shown both interleukin 2 (IL-2) and interferon gamma (IFN) to be elevated in patients with active Crohn's disease compared to ulcerative colitis or non-inflammatory bowel disease controls. However the effect of treatment on these lymphokines has not been studied.
Using a reverse haemolytic plaque assay the percentage of lymphokine-secreting cells was determined in the intestinal mucosa of children with Crohn's disease before and after 8 weeks of treatment with either enteral nutrition, cyclosporin or steroids.
Before treatment, a high percentage of cells isolated from mucosal biopsies secreted IL-2 or interferon-gamma. Eight weeks' treatment with the immunosuppressive agents cyclosporin, or with corticosteroids, produced a significant reduction in the percentage of IL-2 secreting cells, although only for the former was there also a reduction in interferon-gamma secreting cells. Enteral nutrition however, produced a reduction in lymphokine-secreting cells equivalent to cyclosporin and produced the best histological and clinical improvement.
Enteral nutrition and cyclosporin can down-regulate lymphokine secretion in the gut in Crohn's disease.
最近的研究表明，与溃疡性结肠炎或非炎性肠病对照组相比，活动性克罗恩病患者体内的白细胞介素2（IL-2）和干扰素γ（IFN）均升高。然而，尚未研究治疗对这些淋巴因子的影响。
采用反向溶血空斑试验，测定克罗恩病患儿在接受肠内营养、环孢素或类固醇治疗8周前后肠黏膜中分泌淋巴因子的细胞百分比。
治疗前，从黏膜活检中分离出的细胞中有很高比例分泌IL-2或干扰素-γ。用免疫抑制剂环孢素或皮质类固醇治疗8周后，分泌IL-2的细胞百分比显著降低，不过只有前者分泌干扰素-γ的细胞也减少。然而，肠内营养使分泌淋巴因子的细胞减少程度与环孢素相当，并带来了最佳的组织学和临床改善。
肠内营养和环孢素可下调克罗恩病患者肠道中的淋巴因子分泌。
