Breese E, Braegger C P, Corrigan C J, Walker-Smith J A, MacDonald T T
Department of Paediatric Gastroenterology, St Bartholomew's Hospital, London, U.K.
Immunology. 1993 Jan;78(1):127-31.
A sensitive reverse haemolytic plaque assay to detect lymphokine-secreting T cells, and Northern blot analysis to detect expression of lymphokine messenger RNA (mRNA) were used to study interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) production in the mucosa of children with Crohn's disease or ulcerative colitis (UC), and in histologically normal mucosa from patients without inflammatory bowel disease. In the mucosa of most patients with UC and control patients, IL-2- and IFN-gamma-secreting cells were absent or were present at only low levels. In contrast, in mucosa from patients with Crohn's disease, lymphokine-secreting cells were readily detectable (3-18%). IFN-gamma mRNA was detected by Northern blot analysis in 5/6 Crohn's tissues, but only in 1/5 UC samples and none of nine samples of control mucosa. These studies reveal an ongoing cell-mediated immune response in the mucosa in Crohn's disease.
采用一种灵敏的反向溶血空斑试验来检测分泌淋巴因子的T细胞，并运用Northern印迹分析来检测淋巴因子信使核糖核酸（mRNA）的表达，以此研究克罗恩病或溃疡性结肠炎（UC）患儿黏膜中γ干扰素（IFN-γ）和白细胞介素-2（IL-2）的产生情况，同时也研究无炎性肠病患者组织学正常的黏膜。在大多数UC患者和对照患者的黏膜中，未发现分泌IL-2和IFN-γ的细胞，或者其含量仅处于低水平。相比之下，在克罗恩病患者的黏膜中，易于检测到分泌淋巴因子的细胞（3% - 18%）。通过Northern印迹分析，在6份克罗恩病组织中的5份检测到了IFN-γ mRNA，但在5份UC样本中仅1份检测到，而9份对照黏膜样本均未检测到。这些研究揭示了克罗恩病黏膜中持续存在的细胞介导免疫反应。
