Karttunnen R, Breese E J, Walker-Smith J A, MacDonald T T
Department of Paediatric Gastroenterology, St Bartholomew's Hospital, London.
J Clin Pathol. 1994 Nov;47(11):1015-8. doi: 10.1136/jcp.47.11.1015.
To determine the prevalence of cells secreting interleukin-4 (IL-4) in the gut mucosa of children with chronic inflammatory bowel disease.
Mononuclear cells were isolated from intestinal biopsy specimens from control children (n = 10) and children with active inflammatory bowel disease (Crohn's disease n = 15, ulcerative colitis n = 9, indeterminate colitis n = 3). Spontaneous IL-4 production was then measured by SPOT-enzyme linked immunosorbant assay (ELISA) using a pair of non-competing anti-IL-4 monoclonal antibodies. The percentage of T cells in the isolated cells were also determined and the prevalence of IL-4 secreting cells calculated per 10,000 T cells.
In control children the mean number of IL-4 secreting cells was 15.1 per 10,000 T cells. In Crohn's disease and ulcerative colitis the means were 5.3 and 5.2, respectively. In two children with indeterminate colitis numbers were also low. There was no difference in the percentage of T cells in the cell preparations isolated from each patient group. The reduction of IL-4 secreting cells in patients with Crohn's disease was not caused by steroids.
In idiopathic inflammatory bowel disease IL-4 secreting cells are reduced in diseased mucosa.
确定慢性炎症性肠病患儿肠道黏膜中分泌白细胞介素-4（IL-4）的细胞的患病率。
从对照儿童（n = 10）和患有活动性炎症性肠病的儿童（克罗恩病n = 15、溃疡性结肠炎n = 9、未定型结肠炎n = 3）的肠道活检标本中分离单核细胞。然后使用一对非竞争性抗IL-4单克隆抗体，通过斑点酶联免疫吸附测定（ELISA）测量自发IL-4产生。还确定了分离细胞中T细胞的百分比，并计算每10,000个T细胞中分泌IL-4细胞的患病率。
在对照儿童中，每10,000个T细胞中分泌IL-4细胞的平均数为15.1。在克罗恩病和溃疡性结肠炎中，平均数分别为5.3和5.2。在两名未定型结肠炎患儿中，数量也很低。从每个患者组分离的细胞制剂中T细胞的百分比没有差异。克罗恩病患者中分泌IL-4细胞的减少不是由类固醇引起的。
在特发性炎症性肠病中，患病黏膜中分泌IL-4的细胞减少。
