植入前胚胎培养基中的干扰素γ和白细胞介素10水平。
Interferon gamma and interleukin 10 levels in preimplantation embryo culture media.
作者信息
Ozörnek M H, Bielfeld P, Krüssel J S, Moustafa M, Mikat-Drozdzynski B, Koldovsky U, Kuhn U
机构信息
Department of Obstetrics and Gynecology, Frauenklinik, Heinrich Heine Universitat, Düsseldorf, Germany.
出版信息
J Assist Reprod Genet. 1995 Oct;12(9):590-3. doi: 10.1007/BF02212580.
PURPOSE
The aim of our study is to elucidate whether human oocytes/embryos secrete IFN gamma and/or IL-10 and whether the fertilization process depends on the balance between these cytokines.
METHODS
A total of 142 embryo culture media from 24 patients were collected and the cytokine levels were tested with ELISA.
RESULTS
IFN gamma and IL-10 were detectable in 40.1% and 29.6% of culture media respectively. The difference of IFN gamma and IL-10 levels in media from fertilized oocytes between day 1 and day 2 are significant (0.46 vs. 1.47 and 34.2 vs. 12.7, respectively). However there was no significant difference between the IFN gamma levels of the media from fertilized and nonfertilized oocytes 0.46 vs. 0.85 at day 1 and 1.47 vs 1.49 at day 2, as well as IL-10 levels 34.2 vs. 30.9 at day 1 and 12.7 vs. 9.58 at day 2 respectively.
CONCLUSIONS
Human preimplantation embryos secrete the cytokines IFN gamma and IL-10. No effect of these cytokines on fertilization process could be shown.
目的
本研究旨在阐明人类卵母细胞/胚胎是否分泌γ干扰素和/或白细胞介素-10，以及受精过程是否取决于这些细胞因子之间的平衡。
方法
收集了24例患者的142份胚胎培养基，并采用酶联免疫吸附测定法检测细胞因子水平。
结果
分别在40.1%和29.6%的培养基中检测到γ干扰素和白细胞介素-10。受精后第1天和第2天的受精卵培养基中γ干扰素和白细胞介素-10水平差异显著（分别为0.46对1.47和34.2对12.7）。然而，受精和未受精的卵母细胞培养基中γ干扰素水平在第1天为0.46对0.85，第2天为1.47对1.49，白细胞介素-10水平在第1天为34.2对30.9，第2天为12.7对9.58，均无显著差异。
结论
人类植入前胚胎分泌细胞因子γ干扰素和白细胞介素-10。未显示这些细胞因子对受精过程有影响。
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