范特霍夫焓与量热焓之间的显著差异。II.
Significant discrepancies between van't Hoff and calorimetric enthalpies. II.
作者信息
Liu Y, Sturtevant J M
机构信息
Department of Chemistry, Yale University, New Haven, Connecticut 06520, USA.
出版信息
Protein Sci. 1995 Dec;4(12):2559-61. doi: 10.1002/pro.5560041212.
Abstract
Isothermal calorimetric titration of 18-crown-6 ether with BaCl2 in pure aqueous solution over the temperature range 7-40 degrees C gives precise binding constants and enthalpy changes. Nonlinear least-squares fitting of the binding constants to the integrated van't Hoff equation, including a temperature-independent change in heat capacity, leads to van't Hoff enthalpies that differ significantly from the observed calorimetric enthalpies. This perplexing discrepancy appears at present to be very widely occurring.
摘要
在7至40摄氏度的温度范围内，在纯水溶液中用氯化钡对18 - 冠 - 6醚进行等温滴定量热法，可得到精确的结合常数和焓变。将结合常数对积分范特霍夫方程进行非线性最小二乘拟合，包括与温度无关的热容变化，得到的范特霍夫焓与观察到的量热焓有显著差异。目前看来，这种令人困惑的差异非常普遍。
相似文献
1
Significant discrepancies between van't Hoff and calorimetric enthalpies. II.范特霍夫焓与量热焓之间的显著差异。II.
Protein Sci. 1995 Dec;4(12):2559-61. doi: 10.1002/pro.5560041212.
2
Calorimetric vs. van't Hoff binding enthalpies from isothermal titration calorimetry: Ba2+-crown ether complexation.等温滴定量热法测定的量热结合焓与范特霍夫结合焓对比：钡离子与冠醚的络合作用
Biophys Chem. 2004 Jul 1;110(1-2):15-24. doi: 10.1016/j.bpc.2003.12.011.
3
Van't Hoff and calorimetric enthalpies from isothermal titration calorimetry: are there significant discrepancies?范特霍夫焓与等温滴定量热法测得的量热焓：是否存在显著差异？
Biochemistry. 2001 Feb 13;40(6):1774-8. doi: 10.1021/bi002408e.
4
Accounting for apparent deviations between calorimetric and van't Hoff enthalpies.对量热法和范特霍夫焓之间的明显偏差进行解释。
Biochim Biophys Acta Gen Subj. 2018 Mar;1862(3):692-704. doi: 10.1016/j.bbagen.2017.11.020. Epub 2017 Dec 6.
5
The development of a continuous isothermal titration calorimetric method for equilibrium studies.用于平衡研究的连续等温滴定量热法的开发。
Anal Biochem. 2004 Aug 1;331(1):77-88. doi: 10.1016/j.ab.2004.03.022.
6
Disagreements Between Calorimetric and Van't Hoff Enthalpies of Adsorption II: Effect of pH and pH Buffers on Phenobarbital Adsorption to Activated Carbon.量热法和范特霍夫吸附焓的分歧 II：pH 值和 pH 缓冲液对苯巴比妥吸附到活性炭上的影响。
J Pharm Sci. 2023 Jan;112(1):100-107. doi: 10.1016/j.xphs.2022.11.006. Epub 2022 Nov 11.
7
A (partial) resolution of binding enthalpy discrepancies in ITC studies of Ba crown ether complexation: The importance of calibration.在 ITC 研究中 Ba 冠醚络合的结合焓差异的部分解决：校准的重要性。
Anal Biochem. 2022 Apr 1;642:114481. doi: 10.1016/j.ab.2021.114481. Epub 2021 Nov 26.
8
Isothermal acid-titration calorimetry for evaluating the pH dependence of protein stability.用于评估蛋白质稳定性对pH依赖性的等温酸滴定量热法。
Biophys Chem. 2004 May 1;109(2):229-49. doi: 10.1016/j.bpc.2003.11.006.
9
Disagreements Between Calorimetric and Van't Hoff Enthalpies of Adsorption: A New Langmuir-like Model to Account for the Effect of Solvent Displacement Stoichiometry.量热法和范特霍夫吸附焓之间的差异：一种新的类朗缪尔模型，用于解释溶剂置换计量对吸附焓的影响。
J Pharm Sci. 2023 Jan;112(1):91-99. doi: 10.1016/j.xphs.2022.05.003. Epub 2022 May 20.
10
Possible origin of differences between van't Hoff and calorimetric enthalpy estimates.范特霍夫（van't Hoff）与量热法焓估计值之间差异的可能来源。
Biophys Chem. 1997 Feb 28;64(1-3):15-23. doi: 10.1016/s0301-4622(96)02205-3.
引用本文的文献
1
Allostery without Conformational Change: A Native Mass Spectrometry Perspective.无构象变化的变构作用：基于天然质谱的视角
J Phys Chem B. 2025 Aug 28;129(34):8668-8679. doi: 10.1021/acs.jpcb.5c03261. Epub 2025 Aug 19.
2
Sequence Properties of An Intramolecular Interaction That Inhibits p53 DNA Binding.分子内相互作用抑制 p53 DNA 结合的序列特性。
Biomolecules. 2022 Oct 25;12(11):1558. doi: 10.3390/biom12111558.
3
The "Tethered Solvent" Effect - H-Bonding-Controlled Thermo-Halochromism of a Push-Pull Azo Chromophore via Its Secondary Amidoalkyl Acrylamide Side Chain.“束缚溶剂”效应——通过推拉偶氮生色团的二级酰胺基丙烯酰胺侧链控制氢键的热致变色性。
Chemphyschem. 2023 Jan 3;24(1):e202200512. doi: 10.1002/cphc.202200512. Epub 2022 Oct 18.
4
Thermodynamics of semi-specific ligand recognition: the binding of dipeptides to the E.coli dipeptide binding protein DppA.半特异性配体识别的热力学：二肽与大肠杆菌二肽结合蛋白 DppA 的结合。
Eur Biophys J. 2021 Dec;50(8):1103-1110. doi: 10.1007/s00249-021-01572-y. Epub 2021 Oct 5.
5
Interactions of 2'-O-methyl oligoribonucleotides with the RNA models of the 30S subunit A-site.2'-O-甲基寡核糖核苷酸与30S亚基A位点RNA模型的相互作用。
PLoS One. 2018 Jan 19;13(1):e0191138. doi: 10.1371/journal.pone.0191138. eCollection 2018.
6
Experimental support for a desolvation energy term in governing equations for binding equilibria.为结合平衡的控制方程中的去溶剂化能项提供实验支持。
J Phys Chem B. 2013 Jul 11;117(27):8180-8. doi: 10.1021/jp402632a. Epub 2013 Jul 1.
7
Isothermal microcalorimetry to investigate non specific interactions in biophysical chemistry.用等温微量热法研究生物物理化学中的非特异性相互作用。
Int J Mol Sci. 2009 Jul 28;10(8):3283-3315. doi: 10.3390/ijms10083283.
8
Light chain somatic mutations change thermodynamics of binding and water coordination in the HyHEL-10 family of antibodies.轻链体细胞突变改变了HyHEL-10抗体家族中结合的热力学和水配位。
Mol Immunol. 2009 Dec;47(2-3):457-64. doi: 10.1016/j.molimm.2009.08.018. Epub 2009 Sep 24.
9
Thermodynamic analysis reveals a temperature-dependent change in the catalytic mechanism of bacillus stearothermophilus tyrosyl-tRNA synthetase.热力学分析揭示了嗜热脂肪芽孢杆菌酪氨酰 - tRNA合成酶催化机制中与温度相关的变化。
J Biol Chem. 2009 Feb 13;284(7):4179-90. doi: 10.1074/jbc.M808500200. Epub 2008 Dec 20.
10
Structural basis of Clostridium perfringens toxin complex formation.产气荚膜梭菌毒素复合物形成的结构基础。
Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12194-9. doi: 10.1073/pnas.0803154105. Epub 2008 Aug 20.
本文引用的文献
1
Significant discrepancies between van't Hoff and calorimetric enthalpies.范特霍夫焓与量热焓之间存在显著差异。
Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5597-9. doi: 10.1073/pnas.92.12.5597.
2
Rapid measurement of binding constants and heats of binding using a new titration calorimeter.使用新型滴定热量计快速测量结合常数和结合热。
Anal Biochem. 1989 May 15;179(1):131-7. doi: 10.1016/0003-2697(89)90213-3.
3
Test and calibration processes for microcalorimeters, with special reference to heat conduction instruments used with aqueous systems.微量热计的测试与校准过程，特别涉及与水体系一起使用的热传导仪器。
J Biochem Biophys Methods. 1991 Feb-Mar;22(2):101-18. doi: 10.1016/0165-022x(91)90023-p.
Zotero 插件