Einer-Jensen K, Olesen N J, Lorenzen N, Jørgensen P E
Danish Veterinary Laboratory, Aarhus N, Denmark.
Vet Res. 1995;26(5-6):464-9.
Since 1988, VHSV (viral haemorrhagic septicaemia virus) has occasionally been isolated from salmonids and marine fish in USA. The isolates are so far serologically indistinguishable from the European VHSV reference strain F1. However, the nucleotide sequence of an American isolate (Makah) revealed a unique 20 nucleotide sequence in close proximity to the N gene. This sequence is not present in the European VHSV isolates. Through the use of PCR (polymerase chain reaction) and specific primer sets for the amplification of N gene fragments, it is now possible to distinguish between the European and American isolates within half a day.
自1988年以来，美国偶尔会从鲑科鱼类和海鱼中分离出病毒性出血性败血症病毒（VHSV）。到目前为止，这些分离株在血清学上与欧洲VHSV参考毒株F1无法区分。然而，一株美国分离株（马卡）的核苷酸序列显示，在靠近N基因的位置有一段独特的20个核苷酸的序列。该序列在欧洲VHSV分离株中不存在。通过使用聚合酶链反应（PCR）和用于扩增N基因片段的特异性引物组，现在可以在半天内区分欧洲和美国的分离株。
