Southern E M
Department of Biochemistry, University of Oxford, UK.
Electrophoresis. 1995 Sep;16(9):1539-42. doi: 10.1002/elps.11501601256.
Most methods for analysing variation in DNA sequences rely on gel electrophoresis, a method which is difficult to automate and to quantify. Hybridisation to oligonucleotides bound to a solid surface offers alternatives, with the advantage that they can be more readily automated and lend themselves to powerful statistical analytical procedures. In this paper, we discuss a number of different ways in which oligonucleotide arrays could be used for DNA typing for applications in forensic science.
大多数分析DNA序列变异的方法都依赖于凝胶电泳，这是一种难以实现自动化和定量分析的方法。与固定在固体表面的寡核苷酸进行杂交提供了替代方案，其优点是它们更容易实现自动化，并且适用于强大的统计分析程序。在本文中，我们讨论了多种不同的方法，利用这些方法寡核苷酸阵列可用于法医学中的DNA分型。
