Sheridan R L, Schomaker K T, Lucchina L C, Hurley J, Yin L M, Tompkins R G, Jerath M, Torri A, Greaves K W, Bua D P
Department of Surgery, Massachusetts General Hospital, Shriners Burns Institute-Boston Unit 02114, USA.
J Burn Care Rehabil. 1995 Nov-Dec;16(6):602-4. doi: 10.1097/00004630-199511000-00007.
Indocyanine green dye (ICG) fluoresces when illuminated by infrared light. After successful trials in a porcine model and with approval of the Massachusetts General Hospital's human studies committee, 10 adult patients with burn injuries were given 0.2 mg/kg ICG intravenously, and 825 nm fluorescence images were obtained with 780 nm excitation at 5 minutes after injection in the initial five patients and at 1, 2, 3, 4, 5, and 10 minutes in the subsequent five patients. Fluorescence intensities at burned and unburned sites were determined and images were correlated with burn depth as determined by healing or intraoperative assessment. In the latter five patients, seven sites were imaged (six that were of partial thickness and one that was of full thickness). The burn/normal skin fluorescence ratio was greater than 1 for superficial burns and less than 1 for deep burns. Imaging within 5 minutes of injection resulted in optimal contrast between injured and uninjured tissue. In this initial pilot trial it is apparent that ICG fluorescence has potential value as an aid in the early estimation of burn depth. In subsequent trials we will attempt to refine our ability to correlate ICG fluorescence images with burn depth.
吲哚菁绿染料（ICG）在红外光照射下会发出荧光。在猪模型中成功进行试验并获得麻省总医院人体研究委员会的批准后，10名成年烧伤患者静脉注射了0.2mg/kg的ICG，并在最初的5名患者注射后5分钟以及随后的5名患者注射后1、2、3、4、5和10分钟，用780nm激发光获得了825nm的荧光图像。测定烧伤部位和未烧伤部位的荧光强度，并将图像与通过愈合情况或术中评估确定的烧伤深度相关联。在后5名患者中，对7个部位进行了成像（6个为浅度烧伤部位，1个为深度烧伤部位）。浅度烧伤的烧伤/正常皮肤荧光比值大于1，深度烧伤的该比值小于1。注射后5分钟内成像可使受伤组织与未受伤组织之间形成最佳对比度。在这项初步试点试验中，很明显ICG荧光在早期估计烧伤深度方面具有潜在价值。在后续试验中，我们将尝试提高将ICG荧光图像与烧伤深度相关联的能力。
