Enginsu M E, Schütze K, Bellanca S, Pensis M, Campo R, Bassil S, Donnez J, Gordts S
Cliniques Universitaires St-Luc, UCL, Department of Gynecology and Obstetrics, IVF Unit, Brussels, Belgium.
Hum Reprod. 1995 Jul;10(7):1761-4. doi: 10.1093/oxfordjournals.humrep.a136170.
Micromanipulation of mouse gametes with a commercially available compact laser microbeam system was studied. Both the normal in-vitro fertilization (IVF) group and the laser zona dissection (LZD) group were tested under normal (2 x 10(6) motile spermatozoa/ml) and low (500,000 motile spermatozoa/ml) insemination conditions. Subzonal insemination (SUZI) was also tried in a small group of gametes and the results were compared with those of the low insemination groups. Fertilization rates and blastocyst formation rates for the IVF and the LZD-treated groups were respectively 53 and 60% and 60 and 78%, which were not significantly different. However, under low insemination conditions, the results were significantly better in the LZD-treated group (58% fertilization rate and 83% blastocyst formation rate) compared to the results of the IVF group (33 and 48%) (P < 0.05). The SUZI-treated group showed the lowest fertilization rate (18%). No significant difference between the LZD and the IVF group was observed with respect to parthenogenetic activation. LZD has a beneficial effect on fertilization rates in cases of reduced sperm quality.
研究了使用市售紧凑型激光微束系统对小鼠配子进行显微操作。正常体外受精（IVF）组和激光透明带切割（LZD）组在正常（2×10⁶个活动精子/毫升）和低（500,000个活动精子/毫升）授精条件下进行测试。还对一小部分配子尝试了透明带下授精（SUZI），并将结果与低授精组的结果进行比较。IVF组和LZD处理组的受精率和囊胚形成率分别为53%和60%以及60%和78%，差异不显著。然而，在低授精条件下，LZD处理组的结果（受精率58%，囊胚形成率83%）明显优于IVF组（33%和48%）（P<0.05）。SUZI处理组的受精率最低（18%）。在孤雌激活方面，LZD组和IVF组之间未观察到显著差异。LZD对精子质量降低情况下的受精率有有益影响。
