Blann A D, Dobrotova M, Kubisz P, McCollum C N
Department of Surgery, University Hospital of South Manchester, UK.
Thromb Haemost. 1995 Aug;74(2):626-30.
Tissue plasminogen activator antigen (tPA), plasminogen activator inhibitor antigen (PAI-1), soluble P-selectin and von Willebrand factor antigen (vWf) were measured by ELISA in 41 patients with peripheral vascular disease (PVD), 41 with ischaemic heart disease (HD) and in 46 age and sex matched asymptomatic controls. Increased vWf was found in patients with IHD (p = 0.0002) and in patients with PVD (p = 0.0011) relative to the controls but levels did not differ between the two patients groups. Raised tPA found in both PVD (p = 0.0006) and IHD (p = 0.0061) compared to the controls also failed to differentiate the two groups of patients. Soluble P-selectin was also raised in both groups (p = 0.003 in IHD and p = 0.0102 in PVD) with no difference between the groups. There were no differences in levels of PAI-1 between the groups. In the subjects taken as a whole, there were significant Spearman's correlations between tPA and vWf (r = 0.37, p < 0.001), tPA and triglycerides (r = 0.38, p < 0.001), tPA and P-selectin (r = 0.19, p = 0.032), vWf and age (r = 0.25, p = 0.005) and inversely between vWf and HDL (r = -0.25, p = 0.006). These data support the concept that increased levels of tPA may be important in atherosclerosis, and indicate that soluble P-selectin may be useful in further analysis of the role of platelets and the endothelial cell in this disease.
采用酶联免疫吸附测定法（ELISA）检测了41例外周血管疾病（PVD）患者、41例缺血性心脏病（HD）患者以及46例年龄和性别相匹配的无症状对照者的组织型纤溶酶原激活物抗原（tPA）、纤溶酶原激活物抑制剂抗原（PAI - 1）、可溶性P选择素和血管性血友病因子抗原（vWf）。与对照组相比，缺血性心脏病患者（p = 0.0002）和外周血管疾病患者（p = 0.0011）的vWf水平升高，但两组患者之间的水平无差异。与对照组相比，外周血管疾病患者（p = 0.0006）和缺血性心脏病患者（p = 0.0061）的tPA水平升高，同样未能区分这两组患者。两组患者的可溶性P选择素水平也均升高（缺血性心脏病组p = 0.003，外周血管疾病组p = 0.0102），两组之间无差异。各组之间PAI - 1水平无差异。在所有受试者中，tPA与vWf之间存在显著的斯皮尔曼相关性（r = 0.37，p < 0.001），tPA与甘油三酯之间存在显著相关性（r = 0.38，p < 0.001），tPA与P选择素之间存在显著相关性（r = 0.19，p = 0.032），vWf与年龄之间存在显著相关性（r = 0.25，p = 0.005），vWf与高密度脂蛋白呈负相关（r = - 0.25，p = 0.006）。这些数据支持tPA水平升高在动脉粥样硬化中可能起重要作用这一概念，并表明可溶性P选择素可能有助于进一步分析血小板和内皮细胞在该疾病中的作用。
