Brun C, Dijkwel P A, Little R D, Hamlin J L, Schildkraut C L, Huberman J A
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Chromosoma. 1995 Nov;104(2):92-102. doi: 10.1007/BF00347691.
In the budding yeast, Saccharomyces cerevisiae, DNA replication initiates at specific, discrete chromosomal locations. At each initiation site, a single small replication bubble is generated, which subsequently expands at Y-like replication forks. We wanted to know whether other eukaryotic organisms utilize similar initiation mechanisms. For this purpose, replication intermediates (RIs) from three different organisms (Schizosaccharomyces pombe, Chinese hamster and human) were mixed individually with RIs from S. cerevisiae and then subjected to two-dimensional (2D) gel electrophoresis under conditions known to resolve molecules having different structures. All of the RIs detected by the hybridization probes we used for each organism migrated nearly identically to specific RIs of similar size from S. cerevisiae, implying that the detected RIs from all the studied organisms have very similar structures and may therefore employ the same basic initiation mechanism.
在出芽酵母酿酒酵母中，DNA复制起始于特定的、离散的染色体位置。在每个起始位点，会产生一个单一的小复制泡，其随后在Y型复制叉处扩展。我们想知道其他真核生物是否利用类似的起始机制。为此，将来自三种不同生物（粟酒裂殖酵母、中国仓鼠和人类）的复制中间体（RI）分别与酿酒酵母的RI混合，然后在已知可分辨具有不同结构分子的条件下进行二维（2D）凝胶电泳。我们用于每种生物的杂交探针检测到的所有RI迁移情况几乎与来自酿酒酵母的类似大小的特定RI相同，这意味着从所有研究生物中检测到的RI具有非常相似的结构，因此可能采用相同的基本起始机制。
