Pierson L S, Gaffney T, Lam S, Gong F
Department of Plant Pathology, University of Arizona, Tucson, 85721, USA.
FEMS Microbiol Lett. 1995 Dec 15;134(2-3):299-307. doi: 10.1111/j.1574-6968.1995.tb07954.x.
The DNA sequence of five contiguous open reading frames encoding enzymes for phenazine biosynthesis in the biological control bacterium. Pseudomonas aureofaciens 30-84 was determined. These open reading frames were named phzF, phzA, phzB, phzC and phzD. Protein PhzF is similar to 3-deoxy-D-arabino-heptulosonate-7-phosphate synthases of solanaceous plants. PhzA is similar to 2,3-dihydro-2,3-dihydroxybenzoate synthase (EntB) of Escherichia coli. PhzB shares similarity with both subunits of anthranilate synthase and the phzB open reading frame complemented an E. coli trpE mutant deficient in anthranilate synthase activity. Although phzC shares little similarity to known genes, its product is responsible for the conversion of phenazine-I-carboxylic acid to 2-hydroxy-phenazine-I-carboxylic acid. PhzD is similar to pyridoxamine phosphate oxidases. These results indicate that phenazine biosynthesis in P. aureofaciens shares similarities with the shikimic acid, enterochelin, and tryptophan biosynthetic pathways.
测定了生防细菌金黄色假单胞菌30 - 84中编码吩嗪生物合成酶的5个相邻开放阅读框的DNA序列。这些开放阅读框被命名为phzF、phzA、phzB、phzC和phzD。蛋白质PhzF与茄科植物的3 - 脱氧 - D - 阿拉伯庚酮糖 - 7 - 磷酸合酶相似。PhzA与大肠杆菌的2,3 - 二氢 - 2,3 - 二羟基苯甲酸合酶（EntB）相似。PhzB与邻氨基苯甲酸合酶的两个亚基都有相似性，并且phzB开放阅读框补充了一个缺乏邻氨基苯甲酸合酶活性的大肠杆菌trpE突变体。尽管phzC与已知基因的相似性很小，但其产物负责将吩嗪 - I - 羧酸转化为2 - 羟基 - 吩嗪 - I - 羧酸。PhzD与磷酸吡哆胺氧化酶相似。这些结果表明，金黄色假单胞菌中的吩嗪生物合成与莽草酸、肠螯合素和色氨酸生物合成途径有相似之处。
