Wood D W, Pierson L S
Department of Plant Pathology, University of Arizona, Tucson, 85721, USA.
Gene. 1996 Feb 2;168(1):49-53. doi: 10.1016/0378-1119(95)00754-7.
The production of phenazine (Ph) antibiotics in Pseudomonas aureofaciens (Pau) 30-84 is positively regulated by PhzR, a protein belonging to the LuxR family of transcriptional activators. We have now identified phzI, a second gene required for PH production. The product of phzI is a member of the LuxI family of N-acyl-homoserine lactone (N-acyl-HSL) synthases. Inactivation of phzI results in the loss of Ph production in Pau 30-84. The presence of phzI in Escherichia coli is sufficient for the production of a diffusible signal which activates phzB expression in Pau 30-84 and traA expression in a N-acyl-HSL-dependent reporter strain of Agrobacterium tumefaciens. In addition, synthetic N-(3-oxohexanoyl)-L-HSL induces phzB expression in Pau 30-84. These results suggest that Pau 30-84 produces a N-acyl-HSL signal that regulates Ph production, and that phzI plays a central role in this signaling pathway.
在金黄色假单胞菌（Pau）30 - 84中，吩嗪（Ph）抗生素的产生受到PhzR的正向调控，PhzR是一种属于转录激活因子LuxR家族的蛋白质。我们现已鉴定出phzI，这是产生PH所需的第二个基因。phzI的产物是N - 酰基高丝氨酸内酯（N - acyl - HSL）合酶LuxI家族的成员。phzI的失活导致Pau 30 - 84中Ph产生的丧失。phzI在大肠杆菌中的存在足以产生一种可扩散信号，该信号可激活Pau 30 - 84中的phzB表达以及根癌土壤杆菌的N - acyl - HSL依赖性报告菌株中的traA表达。此外，合成的N -（3 - 氧代己酰基）- L - HSL可诱导Pau 30 - 84中的phzB表达。这些结果表明，Pau 30 - 84产生一种调节Ph产生的N - acyl - HSL信号，并且phzI在该信号通路中起核心作用。
