Chernesky M A, Lee H, Schachter J, Burczak J D, Stamm W E, McCormack W M, Quinn T C
McMaster University Regional Virology, St. Joseph's Hospital, Hamilton, Canada.
J Infect Dis. 1994 Nov;170(5):1308-11. doi: 10.1093/infdis/170.5.1308.
A multicenter study compared ligase chain reaction (LCR) of Chlamydia trachomatis plasmid DNA with culture of urethral swab specimens from 542 men (study A); a second study (B) compared LCR of first-void urine (FVU) with urethral swab cultures from 1043 men. Discordant results were resolved with direct fluorescent antibody staining of sediments from the FVU or urethral culture specimen and with a second LCR directed against a fragment of the major outer membrane protein gene. Test performance was calculated on the basis of an expanded reference standard. The LCR plasmid assay had a sensitivity of 98.0% in study A and 93.5% in study B; specificity was 99.8%-100%. The sensitivity of culturing urethral swabs from all study sites was 68.2% (range by sites, 40.0%-84.6%). The presence or absence of urethral symptoms did not alter the results. Use of this LCR test should allow more meaningful investigation and treatment of C. trachomatis infections in men.
一项多中心研究比较了沙眼衣原体质粒DNA的连接酶链反应（LCR）与542名男性尿道拭子标本培养结果（研究A）；另一项研究（B）比较了首次晨尿（FVU）的LCR与1043名男性尿道拭子培养结果。不一致的结果通过对FVU或尿道培养标本沉淀物进行直接荧光抗体染色以及针对主要外膜蛋白基因片段的第二次LCR来解决。基于扩展参考标准计算检测性能。在研究A中，LCR质粒检测的敏感性为98.0%，在研究B中为93.5%；特异性为99.8%-100%。所有研究地点尿道拭子培养的敏感性为68.2%（各地点范围为40.0%-84.6%）。尿道症状的有无并未改变结果。使用这种LCR检测应能对男性沙眼衣原体感染进行更有意义的调查和治疗。
