杆状病毒表达的戊型肝炎病毒结构蛋白的纯化及其在酶联免疫吸附测定中的应用。
Purification of a baculovirus-expressed hepatitis E virus structural protein and utility in an enzyme-linked immunosorbent assay.
作者信息
He J, Ching W M, Yarbough P, Wang H, Carl M
机构信息
Viral and Rickettsial Diseases Program, Naval Medical Research Institute, Bethesda, Maryland, USA.
出版信息
J Clin Microbiol. 1995 Dec;33(12):3308-11. doi: 10.1128/jcm.33.12.3308-3311.1995.
Abstract
We report on the purification of the full-length structural protein encoded by open reading frame 2 (ORF-2) of hepatitis E virus. The ORF-2 protein, expressed in Sf9 cells by using a recombinant baculovirus vector system, was successfully purified to homogeneity. Gel electrophoresis of the purified ORF-2 protein showed a single polypeptide of 75 kDa by Coomassie blue staining and by Western blot (immunoblot) analysis. We demonstrated that the partially purified ORF-2 protein could be used successfully in a sensitive and specific enzyme-linked immunosorbent assay for the detection of antibodies to hepatitis E virus.
摘要
我们报道了戊型肝炎病毒开放阅读框2（ORF-2）编码的全长结构蛋白的纯化。利用重组杆状病毒载体系统在Sf9细胞中表达的ORF-2蛋白成功纯化至均一。纯化后的ORF-2蛋白经考马斯亮蓝染色和蛋白质印迹（免疫印迹）分析，在凝胶电泳中显示为一条75 kDa的单一条带。我们证明，部分纯化的ORF-2蛋白可成功用于灵敏且特异的酶联免疫吸附测定，以检测戊型肝炎病毒抗体。
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