十二烷基硫酸钠在通过rRNA扩增检测结核分枝杆菌中的抑制作用
Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA.
作者信息
Manterola J M, Gamboa F, Lonca J, Matas L, Ruiz Manzano J, Rodrigo C, Ausina V
机构信息
Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Facultad de Medicina, Universidad Autónoma de Barcelona, Badalona, Spain.
出版信息
J Clin Microbiol. 1995 Dec;33(12):3338-40. doi: 10.1128/jcm.33.12.3338-3340.1995.
Abstract
The concentrations of sodium dodecyl (lauryl) sulfate (SDS) found in the sediments of the respiratory samples pretreated with SDS-NaOH varied between 3.36 and 12.42 mg/ml. These concentrations of SDS were higher than the level considered critical (> or = 0.16 mg/ml) to obtain negative results with the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. Extensive washing, after neutralization of sediment, is sufficient to remove all traces of detergent which interfere with the enzymes used by the Mycobacterium Tuberculosis Direct Test assay.
摘要
用SDS-NaOH预处理的呼吸道样本沉积物中十二烷基（月桂基）硫酸钠（SDS）的浓度在3.36至12.42毫克/毫升之间变化。这些SDS浓度高于使用Gen-Probe结核分枝杆菌直接检测法获得阴性结果所认为的临界水平（≥0.16毫克/毫升）。沉积物中和后进行充分洗涤足以去除所有干扰结核分枝杆菌直接检测法所用酶的洗涤剂痕迹。
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